FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1353443
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATATTGCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT58540.4325265267913019No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56290.4159022581667643No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53980.39883467571223913No Hit
AACCAGGACAGCCTCCTAAGGTACTCATTTACTGGGCATCTGCCCGGGCT48210.35620266239509163No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40240.297315808645063No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38570.28497690704373957No Hit
CCTTAGGAGGCTGTCCTGGTTTGTGCTGATACCAAGCTAAGTAGTTCTTGTTGTTGGAACTGAATAAAACACT36410.2690176091641835No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35070.2591169336277922No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33650.24862517298475076No Hit
ATATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCACGCTGTTGATGGTGAGAGTGAAATCTGTC33340.24633471819648112No Hit
GTTTTATTCAGTTCCAACAACAAGAACTACTTAGCTTGGTATCAGCACAA30900.22830662244364927No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30830.2277894229753303No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30820.22771553733699906No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29830.2204008591422025No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29370.21700211977896375No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27950.20651035913592225No Hit
ATATTGCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA27530.20340716232600856No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27190.20089505062274507No Hit
CTCCTAAGGTACTCATTTACTGGGCATCTGCCCGGGCTCCCGGGGTCCCT26510.1958708272162182No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT26150.1932109442362922No Hit
GTCCTGGTTTGTGCTGATACCAAGCTAAGTAGTTCTTGTTGTTGGAACTG26020.19225043093798558No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25940.19165934583133534No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCGCCTGCAAGTCCAGCCAGAGTGTTTTATT25370.18744786444645248No Hit
CCATCAACAGCGTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAA24300.17954210114500574No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC23820.1759955905051044No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23620.17451787773847882No Hit
GGGCAATATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCACGCTGTTGATGGTGAGAGTGAAAT22910.1692719974169581No Hit
GTTTATTACTGTCAACAATATTATATTGCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA22550.16661211443703208No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22190.16395223145710605No Hit
GTAATAAACTGCCACATCTTCAGCCTGCACGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA22110.16336114635045584No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21800.1610706915621862No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21470.15863246549725404No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCGCCTGCAAGTCCAGCCAGAGTGTTTTATTCAGTTCCAA20900.15442098411237118No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20770.15346047081406458No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCGCCTGCAAGTCCAGC20360.1504311596424822No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19670.145333050597624No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19560.14452030857597994No Hit
CACCATCAACAGCGTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATATTGCCCCGCTCACTTT19410.14341202400101075No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19260.14230373942604158No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18930.13986551336110942No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG18340.13550626069956398No Hit
GTCTCTGGGCGAGAGGGCCACCATCGCCTGCAAGTCCAGCCAGAGTGTTTTATTCAGTTCCAACAACAAGAACTA18120.13388077665627587No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATATTGCCCCGC18050.13336357718795694No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17970.1327724920813067No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17760.13122089367634987No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17500.12929986707973665No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC17440.12885655324974896No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17350.12819158250476748No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16990.12553169952484144No Hit
GAATAAAACACTCTGGCTGGACTTGCAGGCGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA16870.12464507186486613No Hit
GTCAGGGACCCCGGGAGCCCGGGCAGATGCCCAGTAAATGAGTACCTTAGGAGGCTGTCCTGGTTTGTGCTGATA16190.11962084845833922No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16120.11910364899002025No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT16050.11858644952170133No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16020.11836479260670749No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15840.11703485111674448No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15790.1166654229250881No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15770.11651765164842554No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15720.11614822345676913No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15620.11540936707345638No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15460.11422719686015591No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15430.11400553994516208No Hit
CTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCG15400.11378388303016825No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCGCCTG15330.11326668356184931No Hit
GATTTCACTCTCACCATCAACAGCGTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATATTGCC15270.11282336973186162No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15260.11274948409353035No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15240.11260171281686779No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15230.11252782717853652No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15130.11178897079522374No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15090.11149342824189863No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15060.11127177132690479No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG14970.11060680058192329No Hit
CCCTTAGACTCTCCTGTGCAGGCTCTGGTTTCACTTTGAAAAATGCCTGG14960.11053291494359202No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14900.11008960111360433No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14670.10839023143198495No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14520.10728194685701578No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14320.1058042340903902No Hit
TCTTCAGCCTGCACGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC14310.10573034845205893No Hit
GCACAAACCAGGACAGCCTCCTAAGGTACTCATTTACTGGGCATCTGCCCGGGCTCCCGGGGTCCCTGACCGATT14250.10528703462207127No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT14190.10484372079208358No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAACAGCG14120.10432652132376466No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13960.1031443511104642No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13820.10210995217382629No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13750.10159275270550737No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13660.10092778196052585No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13660.10092778196052585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3250.039.6153261
CTTATAC6350.039.4839821
CGTATGC6150.038.1739241
TCGTATG6150.038.10500340
ACGCGTA6450.037.84922827
GACGCGT6450.037.83942826
GTATCAA27800.037.6596831
GTATGCC6400.037.28824242
AGACGCG6550.037.24381625
ATCTCGT5350.037.16842337
ATGCCGT6350.037.15639544
TGTCGTA655.649399E-736.98560738
GCCGTCT5950.036.8725946
CGCGTAG6650.036.7353828
GAGACGC6650.036.6756224
GACAGGT1850.036.6286771
CGTAGTA6650.036.27889630
CGAGACG6750.036.12693823
CGCGAGT500.002188960835.72114669
CGAGCCC6950.035.56017715
Produced by FastQC (version 0.11.5)