FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1353443
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATATTGCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT61550.4547661039290166No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61370.45343616243905355No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56680.4187837980616842No Hit
ATATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCACGCTGTTGATGGTGAGAGTGAAATCTGTC43470.3211808698260658No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43230.31940761450611516No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42010.31039356662969925No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40330.2979807793900445No Hit
AACCAGGACAGCCTCCTAAGGTACTCATTTACTGGGCATCTGCCCGGGCT38520.28460747885208315No Hit
CCTTAGGAGGCTGTCCTGGTTTGTGCTGATACCAAGCTAAGTAGTTCTTGTTGTTGGAACTGAATAAAACACT35250.2604468751177552No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32040.23672958521341497No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30550.22572062510205454No Hit
GTAATAAACTGCCACATCTTCAGCCTGCACGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA29890.2208441729721902No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29890.2208441729721902No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28110.2076925293492227No Hit
ATATTGCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA27690.204589332539309No Hit
CTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCG27020.19963899477111338No Hit
GGGCAATATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCACGCTGTTGATGGTGAGAGTGAAAT26930.1989740240261319No Hit
GTTTTATTCAGTTCCAACAACAAGAACTACTTAGCTTGGTATCAGCACAA26650.19690522615285608No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26650.19690522615285608No Hit
GTCCTGGTTTGTGCTGATACCAAGCTAAGTAGTTCTTGTTGTTGGAACTG26550.1961663697695433No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26430.19527974210956797No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26200.1935803724279486No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26000.192102659661323No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25550.1887778059364155No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT25170.18597015167982694No Hit
GTTTATTACTGTCAACAATATTATATTGCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA24030.17754718891006124No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23730.17533061976012287No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23360.1725968511418656No Hit
TCTTCAGCCTGCACGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC23230.17163633784355897No Hit
CTCCTAAGGTACTCATTTACTGGGCATCTGCCCGGGCTCCCGGGGTCCCT22910.1692719974169581No Hit
CCATCAACAGCGTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAA22890.1691242261402955No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC22200.16402611709543732No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCGCCTGCAAGTCCAGCCAGAGTGTTTTATT22150.16365668890378093No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22120.16343503198878712No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21440.15841080858226023No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21190.15656366762397825No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATATTGCCCCGC20560.15190887240910772No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20030.14799293357755No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19730.14577636442761166No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG19520.14422476602265483No Hit
CACCATCAACAGCGTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATATTGCCCCGCTCACTTT19300.1425992819793667No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCGCCTGCAAGTCCAGCCAGAGTGTTTTATTCAGTTCCAA19080.14097379793607856No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18210.13454574740125738No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17910.13232917825131904No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17860.13195975005966265No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG17780.13136866495301242No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17420.12870878197308644No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17390.12848712505809257No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGGAGCCCGGGC17300.12782215431311109No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC16950.12523615697151635No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16860.12457118622653483No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16740.12368455856655951No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCGCCTGCAAGTCCAGC16380.12102467558663349No Hit
GATTTCACTCTCACCATCAACAGCGTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATATTGCC16350.12080301867163966No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15980.11806925005338237No Hit
CCCTTAGACTCTCCTGTGCAGGCTCTGGTTTCACTTTGAAAAATGCCTGG15770.11651765164842554No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15710.11607433781843787No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15530.11474439632847486No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15180.11215839898688013No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14950.11045902930526072No Hit
GTCAGGGACCCCGGGAGCCCGGGCAGATGCCCAGTAAATGAGTACCTTAGGAGGCTGTCCTGGTTTGTGCTGATA14910.11016348675193563No Hit
GTCTCTGGGCGAGAGGGCCACCATCGCCTGCAAGTCCAGCCAGAGTGTTTTATTCAGTTCCAACAACAAGAACTA14900.11008960111360433No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14890.11001571547527306No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14690.1085380027086475No Hit
GAATAAAACACTCTGGCTGGACTTGCAGGCGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA14560.10757748941034087No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGGGCAAT14370.1061736622820466No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14310.10573034845205893No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14240.10521314898373998No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14190.10484372079208358No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14110.10425263568543336No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13940.10299657983380164No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13940.10299657983380164No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13840.10225772345048886No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13810.10203606653549503No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13660.10092778196052585No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13650.10085389632219458No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATTCG200.00797390850.71061320
CGTGCGT450.0093872448.65625470
CGTTTAA450.0093872448.65625470
GTGGTAT3500.045.342691
TGTACCG350.001078331141.2586561
GTATCAA28900.039.724511
TAGCCGC350.001319266439.6011143
GACAGGT2600.038.9605751
CCGTTAA656.417067E-736.38876316
TTCGTCT400.002248491235.52701655
CCGATTA552.2816507E-433.3251765
CCGTTTA550.003203894533.05092669
GAACGCT450.00323081632.9951166
ATCAACG34700.032.8876273
TCAACGC35200.032.4156844
AACGCAG35400.032.2337346
TATCAAC35600.032.1546062
CAACGCA35950.031.8333195
GCCGTTA751.967328E-631.52992815
CGCAGAG37300.030.4107868
Produced by FastQC (version 0.11.5)