FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1312574
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGACTCTCAGGCTGTTCATCTGAAGAAACA59310.4518602379751542No Hit
GTGTATTACTGTGCGAGATCCCCCCTGGGGGGCCGAGAATACTTCCTACA47240.3599035178207095No Hit
GTATTACTGTCAGCATTATGGTGATTCATCGTGGACGTTCGGCCAAGGGA44100.3359810570680205No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32540.24790983213136936No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28390.21629256712383452No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGTCA28180.21469265732827256No Hit
GTGTAGGAAGTATTCTCGGCCCCCCAGGGGGGATCTCGCACAGTAATACACAGCCGTGTCCTCGACTCTCAGG27000.2057026880008289No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26560.20235049604822283No Hit
CTACGCAGACTCCGTGAAGGGCAGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTTTCTTCAGATGAA26430.20136007569858919No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG25200.19198917546744032No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24670.18795130788816478No Hit
CAGTAATACACAGCCGTGTCCTCGACTCTCAGGCTGTTCATCTGAAGAAA24040.18315157850147876No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT23640.1801041312718369No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG22090.16829527325697446No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA21750.16570494311177886No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21120.16090521372509284No Hit
GTATTACTGTGCGAGATCCCCCCTGGGGGGCCGAGAATACTTCCTACACTGGGGCCAGGGCACCCTGGTCACCGT21010.16006716573694132No Hit
GTGTATTACTGTCAGCATTATGGTGATTCATCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA20900.15922911774878978No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19340.14734407355318635No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19250.1466583979265169No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18740.14277290270872348No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18470.14071587582871517No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18150.13827791804500164No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17910.1364494497072165No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT17860.13606851880351126No Hit
CATCTATGCTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGCATCCAGTGCATCTAGCAGGGCGA17580.1339353057427619No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTTTCAGCAGCTACTTAGCCTGGTACC17130.1305069276094148No Hit
GAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGT17010.1295926934405222No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16890.12867845927162963No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGCGTCCAGTGTGAGGTGCAGCTGGTGGAGT16640.12677380475310346No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16540.12601194294569298No Hit
CTTCCTACACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCG16400.12494533641531831No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16320.12433584696938992No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16230.12365017134272048No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15950.12151695828197115No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15940.12144077210123011No Hit
GATCTGCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGG15720.11976467612492706No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTTTCAG15630.11907900049825762No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15480.11793620778714192No Hit
CCATAATGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCT15470.11786002160640086No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGTCA15260.11626011181083887No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT15040.11458401583453581No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15010.11435545729231267No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14820.11290791985823276No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14780.11260317513526856No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14780.11260317513526856No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14560.11092707915896552No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14520.11062233443600133No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC14340.10925098318266246No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT14310.10902242464043933No Hit
GCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTC14220.10833674901376988No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13850.10551786032635113No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC13570.10338464726560177No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCGTGTGCAGC13300.1013276203855935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG19200.051.44791870
CTTATAC5400.043.351191
CGTATGC5650.040.9483541
GTATCAA28250.040.3521351
TCGTATG5650.040.2731340
GGTATCA10900.039.84091
TTATACA6050.039.7969782
TATACAC6050.039.7969783
ATCGTGT459.0803675E-538.97715850
ACGAGAC6100.038.92819222
AACTGTG17500.038.8314570
GTGGTAT3150.038.7737351
ATGCCGT6000.038.73700344
GTATGCC6100.038.5444942
ATCTCGT5300.038.18253737
CATCTCG5400.037.44515636
TACATCT5750.036.26023534
GCCGTCT6150.036.2313146
CCCACGA6600.035.96949419
CCGTCTT6200.035.4468947
Produced by FastQC (version 0.11.5)