FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336930
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT43320.32402594002677776No Hit
GAAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTGATTTGCAGATACA41620.3113102406259116No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA40990.30659795202441414No Hit
GATTATTACTGCTGCTCATATGCGAGTGGTCGCCCCTTTGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT38070.2847568683476323No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG34810.2603726447906771No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC33950.2539399968584743No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33270.2488537170981278No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC32270.24137389392114772No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAAAGGGGCGACCACTCGCATATGAGCAGCAGTAATAAT30240.2261898528718781No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC28250.21130500474968772No Hit
GTTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAA26890.2011324452289948No Hit
GTATATTTCTGTGCGAGGGTGGCTGGTCCGGGATACTCCGGTTTGGACGT26760.2001600682159874No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTATCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG26420.19761692833581415No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC25270.18901513168228704No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAACACTGATATT25240.18879073698697763No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTATCTGGGTCTCCTGGACAGTCGATCAC24870.186023202411495No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAAAGGGGCGACCACTCGCATATGAGCAG24710.18482643070317817No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG23780.17787019514858668No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC23720.1774214057579679No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23340.17457907295071545No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT23190.17345709947416843No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23140.17308310831531942No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22650.16941799495859916No Hit
TACATATACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTG22640.16934319672682938No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22340.16709924977373536No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAAAGGGGCGACCAC21700.16231216294046807No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAAAGGGGCGAC21470.16059180360976266No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21300.15932023366967604No Hit
GTCGATCACCATCTCCTGCACTGGAACCAACACTGATATTGGGAGTTATAACCTTGTCTCCTGGTACCAACAACA21210.15864704958374784No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG20470.15311198043278257No Hit
CTCCTGCACTGGAACCAACACTGATATTGGGAGTTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC20420.15273798927393356No Hit
GACTCAGCCTGCCTCCGTATCTGGGTCTCCTGGACAGTCGATCACCATCT20160.15079323524791874No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG19750.1477265077453569No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTACACATAGCATTAACTGGGTCCGCCAGGCTCCAGGGAAGGGG19120.14301421914385945No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG18950.14174264920377283No Hit
GCATTAACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCTCA18880.14121906158138423No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18580.13897511462829018No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC18000.13463681718564172No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17870.13366444017263432No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT17800.13314085255024571No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17660.1320936773054685No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC17460.13059771267007247No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG17330.12962533565706508No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACAAGGTTATAA17090.12783017809458982No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT16900.12640901169096364No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC16830.12588542406857503No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16710.1249878452873374No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16370.12244470540716418No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC15990.11960237259991174No Hit
GGTTATAACTCCCAATATCAGTGTTGGTTCCAGTGCAGGAGATGGTGATC15960.11937797790460233No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA15760.11788201326920632No Hit
CCCCTGAGGGCCGCTTACTGACCTCAGAAATGATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA15570.11646084686558009No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14940.11174855826408263No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14840.11100057594638463No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGC14820.11085097948284502No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14770.11047698832399601No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14760.11040219009222621No Hit
GCCCTGACTCAGCCTGCCTCCGTATCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAACA14720.11010299716514702No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14700.10995340070160742No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC14570.1089810236886No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14240.10651268204019658No Hit
GTATATGTAAGTACTGGTAGTACTAATGGATGAGACCCACTCCAGCCCCT13540.1012768058163105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4000.053.8632670
GTCGTTT200.00739689151.68329240
TACGCCC200.007639194451.2636635
CGTATGC4450.046.54727641
TCGTATG4350.045.94070440
GGTATCA8850.045.144891
CTTATAC6000.044.016271
TCTCGTA2750.043.68947638
ATGCCGT4900.043.18629544
TGAGCGA6350.042.3340431
CTCTTAT1600.042.3233341
GTATGCC5000.042.19156642
GTCGTAT1550.042.13954539
TTATACA6350.042.107632
CCCACGA6450.041.98739619
TATACAC6450.041.979543
CCGTCTT5150.041.97749747
CTTGAAA5000.041.36757
CTGAGCG6600.041.2057730
GATCTCG3250.041.06365636
Produced by FastQC (version 0.11.5)