FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336930
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60310.4511081358036696No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT38790.2901423410350579No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC37730.28221372846745907No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA37580.281091754990912No Hit
GATTATTACTGCTGCTCATATGCGAGTGGTCGCCCCTTTGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT37400.2797453868190556No Hit
GAAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTGATTTGCAGATACA36240.27106879193375866No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC33880.25341640923608566No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG31980.2392047451998235No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAAAGGGGCGACCACTCGCATATGAGCAGCAGTAATAAT28330.2119033906038461No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTATCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG28220.2110806100543783No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26990.20188042754669278No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTATCTGGGTCTCCTGGACAGTCGATCAC26550.19858930534882155No Hit
GTATATTTCTGTGCGAGGGTGGCTGGTCCGGGATACTCCGGTTTGGACGT25730.1924558503436979No Hit
GTTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAA25500.19073549101299245No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC24310.18183450143238614No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAACACTGATATT24070.1800393438699109No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC23850.17839378277097528No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAAAGGGGCGACCACTCGCATATGAGCAG23710.1773466075261981No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC23320.17442947648717583No Hit
TACATATACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTG21490.1607414000733023No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21350.15969422482852505No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG21310.15939503190144585No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21150.15819826019312905No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21080.15767467257074042No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT21070.15759987433897063No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG21020.15722588318012162No Hit
GTCGATCACCATCTCCTGCACTGGAACCAACACTGATATTGGGAGTTATAACCTTGTCTCCTGGTACCAACAACA20910.15640310263065382No Hit
CTCCTGCACTGGAACCAACACTGATATTGGGAGTTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC20650.154458348604639No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAAAGGGGCGAC20520.15348597159163158No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTACACATAGCATTAACTGGGTCCGCCAGGCTCCAGGGAAGGGG20440.15288758573747316No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19660.1470533236594287No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG19430.14533296432872328No Hit
GACTCAGCCTGCCTCCGTATCTGGGTCTCCTGGACAGTCGATCACCATCT19310.14443538554748564No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18440.13792793938351297No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18240.13643197474811694No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAAAGGGGCGACCAC17780.13299125608670612No Hit
GCATTAACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCTCA17680.1322432737690081No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG17580.1314952914513101No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC17500.13089690559715167No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC17360.1298497303523745No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA17310.12947573919352545No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG17240.12895215157113687No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG17230.12887735333936706No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16920.12655860815450323No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC16880.126259415227424No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16630.12438945943317899No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACAAGGTTATAA16330.12214551248008497No Hit
GCCCTGACTCAGCCTGCCTCCGTATCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAACA15760.11788201326920632No Hit
CCCCTGAGGGCCGCTTACTGACCTCAGAAATGATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA15620.1168348380244291No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15420.11533887338903309No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC15380.11503968046195388No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGC14790.11062658478753562No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14650.1095794095427584No Hit
GGTTATAACTCCCAATATCAGTGTTGGTTCCAGTGCAGGAGATGGTGATC14600.1092054183839094No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14420.10785905021205297No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC13740.1027727704517065No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA13700.10247357752462731No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC13580.1015759987433897No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTATCTGGGTCTCCTGGACAGTCG13410.10030442880330308No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCGA100.0060883714152.5785470
GTGGTAT3500.038.63121
GGTATCA10450.038.16911
GTATCAA24350.037.342171
GACAGGT1700.035.790671
AATGGTA1808.252027E-833.9063470
TGGTATC3850.033.3595622
TATCAAC27850.032.6455962
TCTGGAG17550.032.60225770
TCAACGC27950.032.163614
ATCAACG28000.032.109783
CAACGCA28400.031.652795
GTGCTAG652.669697E-531.2021221
AACGCAG28900.031.1051676
CGCAGAG29950.030.1275028
AGAGTAC30600.029.70397811
CAGAGTA30950.029.4805510
GCAGAGT31250.028.7660589
TATACTG500.0085554327.0357675
ATACTGG1059.2025766E-725.748356
Produced by FastQC (version 0.11.5)