FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1450056
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40030.2760583039551576No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35490.24474916830798257No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT28950.19964746189112698No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28840.19888887049879456No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA28810.19868198193724934No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27420.1890961452523213No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26200.18068267708281613No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT25520.1759932030211247No Hit
GCCTAGAACCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTGGCAAC23480.16192478083605047No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAACCTGAAGATT23040.15889041526672074No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT22880.1577870096051463No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22370.15426990405887772No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21980.15158035275879No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21610.1490287271663991No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21000.1448219930816465No Hit
GTTTATTACTGTCAGCAGCGTGGCAACTGGCCCACGTGGACGTTCGGCCA20920.14427029025085927No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20910.1442013273970109No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20810.14351169885852685No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGTTACTTAGCCTGGTA20630.1422703674892556No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGTTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT20310.1400635561661067No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19950.13758089342756416No Hit
GTAATAGACGGCTGTGTCCTCGATTTTCAGGCTGTTCATTTGCAAATACA19850.13689126488908016No Hit
GTAATAAACTGCAAAATCTTCAGGTTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19690.1357878592275057No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19610.13523615639671846No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19420.1339258621735988No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC19130.13192593941199512No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18610.12833987101187816No Hit
GTGTTAGCAGTTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC18610.12833987101187816No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA18100.12482276546560961No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17760.1224780284347639No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17740.12234010272706711No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAACCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTGGCAACTGG17290.11923677430388895No Hit
GTCAGAGTGTTAGCAGTTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG17130.1181333686423145No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGTGGGCCAGTTGCCACGCTG16740.11544381734222679No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16580.11434041168065234No Hit
GTTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGG16420.11323700601907788No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16260.11213360035750343No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGTCTCACTTTCAGTAACGCCTGG16140.11130604611132261No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16140.11130604611132261No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15900.10965093761896093No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15880.10951301191126411No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15290.10544420353420833No Hit
GTCTATTACTGTACCACAGGAGATTGGGGCTCTGCCGGGGTTTACTTCTCTAACTACGGTTTGGACGTCTGGGGC15230.1050304264111179No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15160.10454768643417908No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15110.1042028721649371No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14980.10330635506490783No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT14960.10316842935721103No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT14910.10282361508796901No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14880.1026167265264238No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14780.10192709798793978No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14680.10123746944945575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAA452.2522963E-644.7449951
GTGGTAT2150.043.7044141
GGTATCA7600.042.831561
CTTATAC6250.039.7335551
TGGTATC2400.039.1262172
TTATACA6600.037.6018182
ATCTCGT5550.037.51610637
TATACAC6750.036.7662283
TCGTATG6200.036.6171140
ATGCCGT6500.036.3272544
CGTATGC6450.035.80966641
CATCTCG6350.033.7912136
GTATCAA17750.033.653281
GTACGGA601.6227545E-533.5587431
TGCTTGA7000.033.33937555
GTATGCC7000.033.08753242
CCCACGA7200.033.07553519
ACGAGAC7050.032.8379322
CTTGAAA7400.032.73116757
TCTACAT7050.032.6526432
Produced by FastQC (version 0.11.5)