FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1450056
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63420.4373624191065724No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41330.28502347495545No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28380.19571657922176797No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT28060.1935097678986191No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA28030.19330287933707388No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27790.1916477708447122No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27020.18633763109838516No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26270.18116541705975492No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAACCTGAAGATT23300.1606834494667792No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGTTACTTAGCCTGGTA21840.15061487280491237No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21520.14840806148176344No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT21230.14640813872015976No Hit
GCCTAGAACCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTGGCAAC20870.14392547598161726No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20800.14344273600467844No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20580.14192555322001357No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20500.14137385038922634No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20290.1399256304584099No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGTTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT20240.13958081618916784No Hit
GTTTATTACTGTCAGCAGCGTGGCAACTGGCCCACGTGGACGTTCGGCCA19760.1362705992044445No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19530.13468445356593126No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19300.13309830792741797No Hit
GTGTTAGCAGTTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC18740.1292363881119074No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC17980.12399521121942876No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAACCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTGGCAACTGG17960.12385728551173197No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17600.12137462277318944No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17590.12130565991934104No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGTGGGCCAGTTGCCACGCTG17490.12061603138085703No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17360.11971951428082776No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA17290.11923677430388895No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17190.11854714576540493No Hit
GTAATAAACTGCAAAATCTTCAGGTTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17150.11827129435001131No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17020.11737477724998208No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16760.11558174304992358No Hit
GTCAGAGTGTTAGCAGTTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG16740.11544381734222679No Hit
GTAATAGACGGCTGTGTCCTCGATTTTCAGGCTGTTCATTTGCAAATACA16740.11544381734222679No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16540.11406456026525871No Hit
GTTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGG16500.11378870884986511No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16460.11351285743447151No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGTCTCACTTTCAGTAACGCCTGG16270.11220256321135184No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15790.10889234622662848No Hit
GTCTATTACTGTACCACAGGAGATTGGGGCTCTGCCGGGGTTTACTTCTCTAACTACGGTTTGGACGTCTGGGGC15750.10861649481123488No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT15730.10847856910353806No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15680.10813375483429606No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15590.10751308914966043No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14860.10247880081872701No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14640.10096161803406214No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14640.10096161803406214No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACT200.006027261754.4298158
TGGTATC2750.042.626422
GTGGTAT2950.040.861951
GGTATCA7950.038.327711
GTATCAA17600.033.1035961
ATACGTA652.8460412E-530.91159611
ATAAACG450.005344342429.770833
ATCGCCC704.285478E-529.11237134
ATCAACG20500.028.7542653
GAATACG704.7126436E-528.7095689
TACGTAC704.7237416E-528.69966512
TCAACGC20750.028.410774
AACGCAG21200.028.280736
TTGAATA500.007395016527.85573445
CAACGCA21300.027.6762035
TATCAAC21200.027.646852
ACGCAGA22050.027.3424477
ACGTACC757.544996E-526.79282413
CGCAGAG22850.026.2385778
AGAGTAC23200.025.9817311
Produced by FastQC (version 0.11.5)