FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126924
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTATTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCAT63140.5602862304822686No Hit
CTCCTAGGCTGCTCATTTACTGGGCCTCTACCCGGCAGTCTGGGGTCCCT56920.5050917364436289No Hit
GCCTAGGAGGCTGTCCTGGTTTCTGCTGATACCAAGCTAAGTAGGTGGTATTGCTGGGGCTGTACAAAACAGT53370.47359005576241164No Hit
GTTTTGTACAGCCCCAGCAATACCACCTACTTAGCTTGGTATCAGCAGAA51370.4558426300265147No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45990.4081020547969517No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT43230.38361060728141383No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41260.3661293929315553No Hit
GTATAATATTGGTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTATC34360.3049007741427106No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31950.2835151261309547No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31690.2812079607852881No Hit
GTACAAAACAGTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA26160.23213632862553288No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26120.23178138011081492No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25640.22752199793419967No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25190.22352882714362282No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTGTA24680.2190032335809691No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23420.20782235536735394No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23330.20702372120923862No Hit
ATACTATTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGA23280.20658003556584115No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22990.2040066588341361No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21920.1945117860654312No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21790.1933582033925979No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21000.1863479702269186No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC19290.17117392122272665No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18220.16167904845402176No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18200.16150157419666278No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTGTACAGCCCCAG17330.15378144400154758No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17240.1529828098434322No Hit
AACCAGGACAGCCTCCTAGGCTGCTCATTTACTGGGCCTCTACCCGGCAG16720.148368479152099No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16710.1482797420234195No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16460.1460613138064324No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16100.14286677717397092No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16070.14260056578793245No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15890.14100329747170173No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15880.14091456034302224No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC15740.13967224054150945No Hit
GCCCCAGCAATACCACCTACTTAGCTTGGTATCAGCAGAAACCAGGACAGCCTCCTAGGCTGCTCATTTACTGGG15710.139406029155471No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15150.13443674994941984No Hit
GTCTGGGGTCCCTGACCGATTCAGTGGCAGCGGCTCTGGGATAGATTTCACTCTCACCATCAGCAGCCTGCAGG13910.12343334599316369No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13660.12121491777617656No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTGTACAGCCCCAGCAATACCACCTA13600.12068249500409967No Hit
GAATAGTATAATATTGGTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT13500.1197951237173048No Hit
ATTCAGTGGCAGCGGCTCTGGGATAGATTTCACTCTCACCATCAGCAGCC13140.11660058708484335No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13090.11615690144144591No Hit
GGGATAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGC13070.11597942718408695No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12910.11455963312521518No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12820.11376099896709983No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12700.112696153422946No Hit
GTCCTGGTTTCTGCTGATACCAAGCTAAGTAGGTGGTATTGCTGGGGCTG12650.11225246777954857No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12510.11101014797803578No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT12420.1102115138199204No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12150.10781561134557432No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12140.10772687421689484No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12100.10737192570217689No Hit
CAGCAATACCACCTACTTAGCTTGGTATCAGCAGAAACCAGGACAGCCTCCTAGGCTGCTCATTTACTGGGCCT12090.1072831885734974No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12070.10710571431613845No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11980.10630708015802308No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11910.10568592025726668No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC11830.1049760232278308No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11740.10417738906971545No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG11740.10417738906971545No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11650.10337875491160008No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11590.10284633213952317No Hit
GTCCCTGACCGATTCAGTGGCAGCGGCTCTGGGATAGATTTCACTCTCAC11580.10275759501084368No Hit
GGATCTCTGGTGCCTGCGGGGACATCATTTTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG11520.10222517223876677No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11440.10151527520933089No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11390.10107158956593346No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11330.10053916679385656No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)