FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126924
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG97050.8611938338344023No Hit
ATATTATACTATTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCAT61950.5497265121694098No Hit
GTATAATATTGGTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTATC59320.5263886473267053No Hit
GCCTAGGAGGCTGTCCTGGTTTCTGCTGATACCAAGCTAAGTAGGTGGTATTGCTGGGGCTGTACAAAACAGT49430.4386276270626946No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47070.41768566469433605No Hit
CTCCTAGGCTGCTCATTTACTGGGCCTCTACCCGGCAGTCTGGGGTCCCT40310.3576993657070042No Hit
GTTTTGTACAGCCCCAGCAATACCACCTACTTAGCTTGGTATCAGCAGAA37720.33471644937901757No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35770.317412709286518No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32820.2912352563260699No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32570.2890168281090828No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26790.23772676773234042No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25870.2295629518938278No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24470.21713975387869988No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24030.21323532021680253No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23170.20560392715036685No Hit
ATACTATTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGA22720.20161075635979No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21400.189897455374098No Hit
GAATAGTATAATATTGGTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT21360.18954250685938004No Hit
GTACAAAACAGTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA21290.18892134695862364No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTGTA20980.1861704959695596No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20810.18466196478200836No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20300.18013637121935464No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18700.16593843063063704No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC18620.16522853360120116No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18490.16407495092836782No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18490.16407495092836782No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18340.16274389399817554No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18200.16150157419666278No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17550.15573366083249626No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16510.1465049994498298No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16330.14490773113359906No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16290.14455278261888113No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15060.13363811579130447No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14980.1329282187618686No Hit
GGGATAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGC14360.1274265167837405No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG14330.12716030539770207No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14280.12671661975430462No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTGTACAGCCCCAG14000.12423198015127906No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13830.12272344896372782No Hit
ATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTATCCCAGAGCCGCTGCCA13280.11784290688635612No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13240.11748795837163818No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTATCCCAGAGCCGCTGCCACTGAATCGGTCAGGGACC13190.11704427272824078No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13080.11606816431276644No Hit
CTCCTGTGCAGTCTCTGGATTTACTTTCGCTGCCTACTGGATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGG12610.11189751926483063No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12560.11145383362143321No Hit
CAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTATCCCAGAGCCGCTGCCA12550.11136509649275372No Hit
GTCTGGGGTCCCTGACCGATTCAGTGGCAGCGGCTCTGGGATAGATTTCACTCTCACCATCAGCAGCCTGCAGG12530.11118762223539475No Hit
GAGTGAAATCTATCCCAGAGCCGCTGCCACTGAATCGGTCAGGGACCCCA12320.10932414253312557No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12190.10817055986029227No Hit
GTCCTGGTTTCTGCTGATACCAAGCTAAGTAGGTGGTATTGCTGGGGCTG12060.10701697718745895No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC12000.10648455441538206No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGAGGAATAGT11950.10604086877198463No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11940.10595213164330514No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11910.10568592025726668No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11900.1055971831285872No Hit
GCCGGGTAGAGGCCCAGTAAATGAGCAGCCTAGGAGGCTGTCCTGGTTTC11880.10541970887122823No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11690.10373370342631802No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT11530.10231390936744625No Hit
AACCAGGACAGCCTCCTAGGCTGCTCATTTACTGGGCCTCTACCCGGCAG11390.10107158956593346No Hit
GGATCTCTGGTGCCTGCGGGGACATCATTTTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG11390.10107158956593346No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11350.10071664105121551No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11310.10036169253649757No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)