FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290268
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT22530.7761792550332796No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA8640.29765595932035227No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT8550.29455537641076524No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG7740.2666501302244822No Hit
GACCTAGGACGGTCACCTCGGTCCCAGTTCCGAAGACCTGAATGCCAATGCCCCACGTCTGGCAGTAATAGTCAG7180.24735761434260753No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC7030.24218997615996252No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC6550.2256535339754985No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC6350.21876334973197184No Hit
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA6150.21187316548844518No Hit
GGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC5800.1998153430622735No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTCGGTCCCAGTTCCGAAGACCTG5440.18741301142392547No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC5360.1846569377265148No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG5220.17983380875604613No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCT5210.1794892995438698No Hit
GACTATTACTGCCAGACGTGGGGCATTGGCATTCAGGTCTTCGGAACTGGGACCGAGGTGACCGTCCTAGGTCAG4980.17156558766381413No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTCACCTCGGTC4930.16984304160293245No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT4880.16812049554205077No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC4820.1660534402689928No Hit
CAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGG4810.16570893105681644No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG4790.1650199126324638No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT4780.16467540342028744No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT4720.16260834814722944No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT4670.16088580208634778No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC4400.1515840533575868No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT4360.15020601650888146No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCAGGTACGCCATCGCATGGCATC4290.14779445202364713No Hit
GTAATAGTCAGCCTCATCCTCAGGCTGGAGACTGGAGATGGTGAGGTAGC4130.14228230462882577No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGTCTCCAGCCTGAGGAT4110.14159328620447312No Hit
CCTGCACTCTGAGCAGTGGGCACAGCAGGTACGCCATCGCATGGCATCAG4090.14090426778012044No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA4060.13987074014359144No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG3990.1374591756583571No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG3910.13470310196094643No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA3900.1343585927487701No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTACCACCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGA3880.13366957432441742No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG3800.13091350062700677No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC3790.13056899141483044No Hit
ATATATTACTGTGCCAAATTACCCAGGGGCGGGGGGCCAACACCTGACACCGGAGGGTACTGGGGCCAGGGAACC3710.12781291771741976No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC3620.12471233480783275No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA3600.12402331638348009No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT3550.12230077032259842No Hit
GGATGAGGCTGACTATTACTGCCAGACGTGGGGCATTGGCATTCAGGTCTTCGGAACTGGGACCGAGGTGACCGT3520.12126724268606943No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG3440.11851116898865875No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG3270.11265451238166109No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA3250.11196549395730841No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT3210.11058745710860308No Hit
CTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT3140.10817589262336876No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG3130.1078313834111924No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTG3060.10541981892595807No Hit
ACCTAGGACGGTCACCTCGGTCCCAGTTCCGAAGACCTGAATGCCAATGCCCCACGTCTGGCAGTAATAGTCAGC3060.10541981892595807No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG3050.10507530971378175No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG3030.10438629128942907No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC3020.10404178207725275No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT2980.10266374522854742No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGT100.006586413148.574870
AGGGGGG2550.0104.8763370
CCGTCGT207.891405E-570.0477447
CGCGTAT150.002397563868.6795739
ATCGCGT150.002441363668.3667937
ACCTAAT150.002599374267.2941361
CCTAATA150.0026064967.247762
TAGTTCA200.00567056255.26326464
ATGGGGG701.4612868E-553.06242870
CGTCGTC200.0068494852.68709648
AAGGGTA200.0068494852.68709648
GATACTT200.007319411751.8105341
CGTATGG200.007319411751.8105341
AATGTCG200.007684637351.17648736
AATCGCG200.007684637351.17648736
CTAATCG200.00778070451.01594534
CTCTTAT1200.050.47061
GACTTAC200.008146157550.427137
CTTGAAA3850.049.63092857
TATCGTA352.373397E-548.91052638
Produced by FastQC (version 0.11.5)