FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290268
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47751.645031488141993No Hit
GGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC8930.3076467264734659No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT8020.27629638816541957No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC7660.26389405652707154No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA7050.2428789945843152No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC7010.24150095773560984No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG6580.22668706161202748No Hit
GACCTAGGACGGTCACCTCGGTCCCAGTTCCGAAGACCTGAATGCCAATGCCCCACGTCTGGCAGTAATAGTCAG6330.21807433130761913No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC5750.19809279700139182No Hit
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA5390.18569046536304382No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTCGGTCCCAGTTCCGAAGACCTG4990.17191009687599046No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT4880.16812049554205077No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC4870.16777598632987445No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT4770.16433089420811112No Hit
GACTATTACTGCCAGACGTGGGGCATTGGCATTCAGGTCTTCGGAACTGGGACCGAGGTGACCGTCCTAGGTCAG4750.16364187578375844No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG4640.15985227444981878No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCT4530.15606267311587912No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG4500.15502914547935012No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT4440.15296209020629212No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG4390.15123954414541046No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC4340.14951699808452878No Hit
CTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT4280.14744994281147078No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTCACCTCGGTC4260.14676092438711813No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT4200.14469386911406013No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT4130.14228230462882577No Hit
ATATATTACTGTGCCAAATTACCCAGGGGCGGGGGGCCAACACCTGACACCGGAGGGTACTGGGGCCAGGGAACC3890.13401408353659378No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA3870.1333250651122411No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTACCACCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGA3860.13298055590006477No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGTCTCCAGCCTGAGGAT3810.1312580098391831No Hit
CAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGG3800.13091350062700677No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCAGGTACGCCATCGCATGGCATC3800.13091350062700677No Hit
GTAATAGTCAGCCTCATCCTCAGGCTGGAGACTGGAGATGGTGAGGTAGC3680.12677939008089076No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC3660.12609037165653808No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG3610.12436782559565643No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA3540.12195626111042208No Hit
GGATGAGGCTGACTATTACTGCCAGACGTGGGGCATTGGCATTCAGGTCTTCGGAACTGGGACCGAGGTGACCGT3530.12161175189824576No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG3520.12126724268606943No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA3520.12126724268606943No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG3480.1198892058373641No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT3450.1188556782008351No Hit
CCTGCACTCTGAGCAGTGGGCACAGCAGGTACGCCATCGCATGGCATCAG3440.11851116898865875No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC3430.11816665977648243No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTG3160.1088649110477214No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGC3140.10817589262336876No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT3140.10817589262336876No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT2970.10231923601637108No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG2920.10059668995548941No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)