FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1337331
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAACAGTGATTCGTGGACGTTCGGCCAGGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCAT63810.4771444017973112No Hit
CTGTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC62290.4657784796733195No Hit
GTAATAGACGCCCGTGTCCTCGGCTGTCAGGCTGTTCATTTGCAGATAGA41490.3102448085029062No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38780.28998056576868403No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34870.26074322662078425No Hit
ATTAATAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGCCAAGCCCCTAA34230.25595757520015616No Hit
CAGTATAACAGTGATTCGTGGACGTTCGGCCAGGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCAT30100.22507516837641542No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGATCTGGCTTTTT27550.2060073384973503No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26940.20144601448706415No Hit
CCTATTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA26260.1963612598526468No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25750.19254769387683376No Hit
GTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC25500.19067829879065093No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24510.18327549424936684No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24240.18125654755628934No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23740.17751775738392364No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23320.17437717363913646No Hit
CCCTAAGACTCTCCTGTTCAGCTTCTGGATTCACCTTTAGCAGCTATTTT22770.1702645044495342No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22770.1702645044495342No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA22580.1688437641840352No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT21950.16413288856685443No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20890.15620665340143913No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC20740.1550850163497294No Hit
GTCTATTACTGTGCGAAAGGGGACTCCCCCTCACTTCCAATTGCCCTTGGGGGCCAGGGAACCCTGGTCACCGT20310.15186965680149492No Hit
GTCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC20300.1517948809980476No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20210.15112189876702176No Hit
CAGTAATAGACGCCCGTGTCCTCGGCTGTCAGGCTGTTCATTTGCAGATA20040.14985071010841744No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20040.14985071010841744No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20040.14985071010841744No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG19450.1454389377050259No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19040.14237312976368605No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19030.1422983539602387No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAATAGGTGGTTGGCCTGGTATCAGCAGAAA18620.13923254601889884No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18470.1381109089671891No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18430.13781180575339985No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT18040.13489554941895462No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17970.13437211879482341No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA17120.12801617550180172No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17070.12764229648456515No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCCTGGCCGAACGTCCACGAATCA16240.12143590479843809No Hit
TCTATAAGGCGTCTACTTTACAAACTGGGGTCCCATCGAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT16110.120463819353623No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15880.11874397587433476No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15870.11866920007088747No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15800.11814576944675625No Hit
CTCCTGTTCAGCTTCTGGATTCACCTTTAGCAGCTATTTTATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG15730.11762233882262507No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15480.11575294373644221No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTCTCCCAGGTGCCAAATGTGACAT15240.11395832445370668No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15150.11328534222268084No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14930.11164027454683993No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14900.111415947136498No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14880.11126639552960338No Hit
GGTTAGGGGCTTGGCCTGGTTTCTGCTGATACCAGGCCAACCACCTATTA14490.10835013919515812No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATAAAATAGCTGC14330.1071537263400011No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14230.10640596830552794No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14060.10513477964692361No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14000.10468612482623972No Hit
CTATTACTGTGCGAAAGGGGACTCCCCCTCACTTCCAATTGCCCTTGGGGGCCAGGGAACCCTGGTCACCGTCTC13740.10274195393660955No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13670.10221852331247837No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13650.10206897170558374No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13510.10102211045732133No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13450.10057345563663746No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13400.10019957661940088No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG13400.10019957661940088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT2100.048.9446437
CGTATGC2100.047.591841
TCGTATG1900.047.09372740
CGCGTAT451.9115232E-645.823639
ATTCGCT306.656465E-445.5063333
CTACGCT4950.045.3425328
ACGCTGC4950.044.69909730
TACGCTG5050.044.46299729
ACTACGC5000.044.1758427
ATGCCGT2600.043.96035444
ACGAGAC5050.042.99629222
TCTGGAG15650.042.8655670
CACGAGA5200.041.75288821
CGAGACT5150.041.50884623
CTTATAC5900.041.4021451
ATCTCCG5500.041.31041710
TATACAC5850.041.1591343
TGCATCG1750.040.99117334
GTATCAA29400.040.735191
GAGACTA5350.040.5974324
Produced by FastQC (version 0.11.5)