FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1337331
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC66340.4960626800694817No Hit
GTATAACAGTGATTCGTGGACGTTCGGCCAGGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCAT60670.45366479951485456No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45730.34194974916456733No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39480.2952148720099961No Hit
GTAATAGACGCCCGTGTCCTCGGCTGTCAGGCTGTTCATTTGCAGATAGA34930.2611918814414681No Hit
ATTAATAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGCCAAGCCCCTAA30260.22627158123157246No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28440.2126623850041613No Hit
CAGTATAACAGTGATTCGTGGACGTTCGGCCAGGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCAT28060.20982090447316334No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26620.19905318877675013No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26120.19531439860438438No Hit
GTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC25950.19404320994578006No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGATCTGGCTTTTT24320.18185475398386788No Hit
CCTATTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA24110.18028446211147428No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22810.17056360766332346No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21590.16144095964275113No Hit
CCCTAAGACTCTCCTGTTCAGCTTCTGGATTCACCTTTAGCAGCTATTTT21290.1591976855393317No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA21280.1591229097358844No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21190.15844992750485856No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20810.15560844697386061No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20380.1523930874256261No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20120.15044891653599596No Hit
GTCTATTACTGTGCGAAAGGGGACTCCCCCTCACTTCCAATTGCCCTTGGGGGCCAGGGAACCCTGGTCACCGT20090.150224589125654No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19780.14790653921878727No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC19140.14312088779815918No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19120.14297133619126454No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19080.1426722329774753No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTCGATGGGACCCCA18940.14162537172921288No Hit
GTCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC18180.13594241066721702No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17910.13392346397413954No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17790.13302615433277176No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17560.13130631085348354No Hit
CAGTAATAGACGCCCGTGTCCTCGGCTGTCAGGCTGTTCATTTGCAGATA17370.12988557058798456No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA17200.12861438192938024No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17100.1278666238949071No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAATAGGTGGTTGGCCTGGTATCAGCAGAAA16960.1268197626466447No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16800.12562334979148768No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCCTGGCCGAACGTCCACGAATCA16700.12487559175701453No Hit
CTCCTGTTCAGCTTCTGGATTCACCTTTAGCAGCTATTTTATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG16320.1220341112260166No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG16270.12166023220878003No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16220.12128635319154345No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16090.12031426774672838No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15680.11724845980538849No Hit
GGGCTGGAGTGGGTCGCAGCAATTAGTGCTCGTACTGGTGGCACATACTTCGCAGACTCCGTGAAGGGCCGGTT15640.11694935659159925No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT15490.11582771953988953No Hit
TCTATAAGGCGTCTACTTTACAAACTGGGGTCCCATCGAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT15050.11253758418820771No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15010.11223848097441846No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14810.11074296490547217No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14750.1102943100847883No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14450.10805103598136886No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14140.10573298607450213No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14080.10528433125381823No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14080.10528433125381823No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13970.1044617974158978No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTCTCCCAGGTGCCAAATGTGACAT13900.10393836679176659No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATAAAATAGCTGC13890.10386359098831927No Hit
GGTTAGGGGCTTGGCCTGGTTTCTGCTGATACCAGGCCAACCACCTATTA13570.1014707652780052No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13510.10102211045732133No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13480.10079778304697941No Hit
CTATTACTGTGCGAAAGGGGACTCCCCCTCACTTCCAATTGCCCTTGGGGGCCAGGGAACCCTGGTCACCGTCTC13470.10072300724353207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTT1000.051.78585468
CACCGTT1000.045.19807467
CGGTTAC350.001473473938.72040620
CCTAACG350.001485176638.6581272
GTATCAA29100.038.478341
TCTGGAG15150.037.4261670
CGCACAC1150.037.0089856
GTGTATA754.7813955E-836.0836141
GTGGTAT3600.035.7077451
GCACCGT759.569558E-734.5765666
AATGGTA1955.2805262E-934.43358270
CCGCACA1251.8189894E-1233.96832755
TATCAAC34750.032.414422
ATCAACG35750.031.416633
TCAACGC35700.031.2734494
AACGCAG35900.030.9107446
CAACGCA36250.030.7056265
CATATGT604.4816048E-429.70139158
ACGCAGA37950.029.4192927
AGAGTAC38600.029.178111
Produced by FastQC (version 0.11.5)