FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077902
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT38840.3603296032477906No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC34900.32377711517373564No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA33540.3111600126913207No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32040.29724409083571607No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG31540.29260545021718115No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC28910.2682062005636876No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC27520.2553107796441606No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGAGCCAGTGCCCCAGGTCTGACAGTAATAGTCAG26850.24909500121532385No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC25910.2403743568524782No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC24620.2284066640566582No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22880.21226419470415678No Hit
GCTTAACAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCT22810.21161478501756187No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATACA22480.20855328220932887No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT22370.2075327812732512No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCTCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAG22350.20734723564850976No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGC22250.20641950752480284No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA22050.20456405127738886No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGAGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGC21890.20307968627945766No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21340.1979771815990693No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCT20690.19194694879497395No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG20140.18684444411458556No Hit
CTGTTAAGCTTCATCAAGTATCGAGGGCCCTTCTCTGGCTGCTGCTGATGCCATGCGATGGCGTAGCTGCTGTGC19400.17997925599915393No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC19210.17821657256411064No Hit
GGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC18510.17172247569816182No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG18300.16977424663837715No Hit
CTATTACTGTCAGACCTGGGGCACTGGCTCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCC18250.16931038257652367No Hit
CCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAG18020.16717660789199762No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG17870.16578501570643714No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG17230.15984755571471246No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG16840.15622941603225524No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT16640.15437395978484128No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16320.15140522978897897No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATC15890.14741599885703893No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGAC15560.14435449604880593No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC15410.14296290386324545No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGC15280.14175685730242638No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC15230.14129299324057287No Hit
GTATATTACTGTGCGAAATCGCCTACGGTGGTAACCTACTGGGGCCAGGG14900.13823149043233987No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA14200.131737393566391No Hit
CAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAG13970.12960361888186495No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC13620.12635657044889054No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA13370.12403725013962308No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGCTCTTATGTCTTCGGAACTGGGACCAAGGT13340.12375893170251098No Hit
CTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT12870.11939860952108819No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC12820.1189347454592347No Hit
TCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC12770.1184708813973812No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA12720.11800701733552772No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA12680.11763592608604492No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG12590.11680097077470865No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12430.11531660577677749No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12280.11392501359121701No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12180.11299728546751003No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11800.10947191859742353No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT11780.10928637297268212No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT11690.10845141766134585No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG11510.10678150703867328No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT11420.10594655172733698No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC11360.1053899148531128No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11090.10288504891910397No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC11030.10232841204487977No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10900.1011223654840607No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT10810.10028741017272443No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10780.10000909173561233No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)