FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1519545
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC43660.28732284993205204No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA28700.1888723269136485No Hit
GCCTTGGGCTGACCGAGGACGGACAGTTTGGTCCCTCCGCCGAAAATTCC27760.18268626463842794No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG27220.17913256928883317No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT26910.1770924849214732No Hit
CTCCAGAGACAATTCCAAGAACACAGTTTATCTGCAAATGAACAGCCTGAGAGTTGAGGACTCGGCTGTCTACTT26880.17689505740205128No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26670.17551306476609774No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG26270.172880697840472No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25440.16741853646979854No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24650.16221961179168765No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT22390.1473467386619021No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG22280.146622837757355No Hit
GTCTACTTCTGTGGGAAAGATTGGGGTCGTGGCATACCAGCAAATGGCCCTCCCCCCTTTGGGGACTCCTGGGGC22070.14524084512140148No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21990.14471437173627633No Hit
GAAGTAGACAGCCGAGTCCTCAACTCTCAGGCTGTTCATTTGCAGATAAA21830.14366142496602602No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21580.14201619563750992No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC21150.1391864011924622No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT21090.13879154615361836No Hit
GAGTTTGGACTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAAT21020.13833088194163384No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA20490.1348429957651797No Hit
CTATTATTGTCAGACGTGGGCCAGCAGCACTGGAATTTTCGGCGGAGGGACCAAACTGTCCGTCCTCGGTCAGCC20480.13477718659203908No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20210.13300033891724167No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20030.13181577380071008No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCGCGCCAAAATTAC19940.13122349124244428No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT18930.12457676475523924No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAAACA18830.12391867302383279No Hit
GCACTGAACACGGAGGACTCACCATGGAGTTTGGACTGAGCTGGGTTTTC18600.12240506204159798No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18560.12214182534903541No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGACAGTTTGGTCCCTC18300.12043078684737865No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18150.119443649250269No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT17720.1166138548052213No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17530.11536348051554907No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17250.11352082366761103No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT17100.11253368607050138No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG17070.11233625855107943No Hit
CCTTGGGGCTGGTCGGGGATGCCGAGGAGACGGTGACCTGGGTTCCCTGG17060.1122704493779388No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT17030.11207302185851685No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16460.10832189898950015No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16190.10654505131470277No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16180.10647924214156211No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC16070.10575534123701504No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15790.103912684389077No Hit
CCCTGAGACTCTCCTGTGAGGCCTCGGGATTCACCTTCAGTAATTTTGGC15480.10187260002171702No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC15300.10068803490518544No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15290.1006222257320448No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15240.10029317986634156No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAC200.006235475753.96471864
CTAACGC409.0069625E-751.1111073
CGTATGC3750.042.35218441
TCTCGTA3250.042.33292838
TATCTCG3300.041.6058436
GCCTATC3750.041.09306733
TCGTATG3750.040.4689640
ACGAGAC4350.039.9620622
ATGCCGT4250.039.95192344
CCGCGTG350.001432824638.941799
ATCTCGT3500.038.28445437
CCTATCT3900.037.77643634
AGCCTAT4350.037.7654932
GCCGTCT4200.037.22449546
GAGACCG4500.036.3636224
TATACAC5300.036.0027923
TCTCCGA4750.035.86744311
GACCGGA4600.035.59537526
TTATACA5300.035.3598862
GAGCCTA4950.035.24579231
Produced by FastQC (version 0.11.5)