FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1519545
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49440.3253605520073443No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC39390.259222333000997No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28180.185450249910335No Hit
CTCCAGAGACAATTCCAAGAACACAGTTTATCTGCAAATGAACAGCCTGAGAGTTGAGGACTCGGCTGTCTACTT27160.1787377142499893No Hit
GCCTTGGGCTGACCGAGGACGGACAGTTTGGTCCCTCCGCCGAAAATTCC25150.16551007044871985No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC23880.15715230545985803No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG23790.15656002290159224No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23520.15478317522679486No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22640.1489919679904182No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22410.14747835700818338No Hit
GAGTTTGGACTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAAT21870.1439246616585886No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21840.14372723413916666No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21580.14201619563750992No Hit
GTCTACTTCTGTGGGAAAGATTGGGGTCGTGGCATACCAGCAAATGGCCCTCCCCCCTTTGGGGACTCCTGGGGC21350.1405025846552751No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA20670.1360275608817113No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC20630.13576432418914872No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA19330.127209131680865No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18810.12378705467755151No Hit
CTATTATTGTCAGACGTGGGCCAGCAGCACTGGAATTTTCGGCGGAGGGACCAAACTGTCCGTCCTCGGTCAGCC18710.12312896294614506No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT18700.12306315377300442No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCGCGCCAAAATTAC18660.12279991708044184No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18120.11924622173084708No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG18100.11911460338456578No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT17860.11753518322919032No Hit
GAAGTAGACAGCCGAGTCCTCAACTCTCAGGCTGTTCATTTGCAGATAAA17590.11575833555439294No Hit
GCACTGAACACGGAGGACTCACCATGGAGTTTGGACTGAGCTGGGTTTTC17160.11292854110934523No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16950.1115465484733917No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAAACA16680.10976970079859431No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG16560.1089799907209066No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGACAGTTTGGTCCCTC16550.10891418154776594No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16500.10858513568206271No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16480.10845351733578144No Hit
CTCCTGTGAGGCCTCGGGATTCACCTTCAGTAATTTTGGCGCGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG16330.10746637973867176No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16260.10700571552668726No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16160.10634762379528083No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16150.10628181462214019No Hit
GCCTGAGAGTTGAGGACTCGGCTGTCTACTTCTGTGGGAAAGATTGGGGTCGTGGCATACCAGCAAATGGCCCTC16080.10582115041015568No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15790.103912684389077No Hit
CCTTGGGGCTGGTCGGGGATGCCGAGGAGACGGTGACCTGGGTTCCCTGG15630.10285973761882669No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15470.1018067908485764No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT15380.10121450829031059No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC200.00778952851.0119312
CTAACGC750.049.886543
TAACGCC950.046.5463874
CCTAACG903.6379788E-1241.578962
GTGGTAT4450.038.2199861
ATGTACC1052.0008883E-1135.6273811
CACGATT400.002600518534.48935340
GTATTAC502.0229038E-434.015791
ATCAACG45350.033.151023
AACGCAG45500.032.8933076
ACGCCAT1155.638867E-1132.535776
TCAACGC46400.032.475214
CAACGCA46800.032.0522845
CCAATAC851.3693352E-732.0127533
CGGAAAT1101.1823431E-931.04508830
ACGCAGA48250.031.0185597
CGCAGAG48050.030.8645088
GTATCAA50650.030.8257581
TTCGATC902.0307561E-730.60611239
CGGGTAT450.00494369930.24920317
Produced by FastQC (version 0.11.5)