FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179038
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT18341.0243635429350193No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC4260.2379383147711659No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC4120.23011874574112756No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT3570.19939901026597706No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT3410.19046235994593327No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT2850.15918408382578No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC2720.15192305544074444No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT2720.15192305544074444No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA2700.15080597415073896No Hit
TCTCTGGACTCCAGCCTGACGATGAGGCTGATTATTACTGCAGTTCATAT2660.148571811570728No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC2490.13907662060568146No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG2400.13404975480065684No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC2390.13349121415565413No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC2380.13293267351065136No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC2330.13013997028563767No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG2240.12511310448061305No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC2240.12511310448061305No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC2040.11394229158055832No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT1900.10612272255052001No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT1890.10556418190551727No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG1860.10388855997050905No Hit
CCCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTT1800.10053731610049263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2350.096.6662670
CGTGCAA251.9662952E-458.23071757
CGGTACC252.3458277E-456.18904545
ATGCCCG351.41766195E-553.34362862
ACTGTAC253.0493672E-453.2846073
ATTGTAG200.006668154653.0366447
CGTCTTC3600.052.2404448
ATCTCGT3250.051.4909337
CTTGAAA3400.051.38004357
GCCGTCT3650.051.15928346
CATAATA200.00792127250.77719534
AGATCTC3350.050.68291535
GCTTGAA3450.050.48126656
CTTATAC3450.050.237751
ATGCCGT3700.050.09773344
CCGACTT200.008459249549.94036512
TCTCGTA3500.049.89322738
TCGTATG3700.049.48784640
AAAGGGG2800.048.97865368
CGTATGC3700.048.72358341
Produced by FastQC (version 0.11.5)