FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1373061
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCTGTATGGAAGTACATTCTGGTCGTTCGGCCAAGGGA70710.5149807619617774No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55520.4043520280599332No Hit
GTCTATGTCTCCCGGGGACAGAGCCACCCTCTCCTGCAGGGCCAGTGAGA50100.3648781809402496No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44380.32321943453349855No Hit
CAGTAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTACCA44030.3206703853652533No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCA38520.28054106845944937No Hit
GTGTATTACTGTCAGCTGTATGGAAGTACATTCTGGTCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA37350.27201996123988664No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34610.2520645477513381No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34100.24835021896332357No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29150.2122993807267121No Hit
GTAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTACCA27880.20304997374479355No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26700.19445603654899526No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25750.1875371888066153No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25680.18702737897296626No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25460.18542511949578352No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24960.18178362068400458No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24870.18112815089788437No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT24860.18105532092164878No Hit
CTCCTGCAGGGCCAGTGAGAGTGTCAACAGCAGGTACTTAGCCTGGTACC24690.17981721132564393No Hit
GTCTCCAGGCACCCTGTCTATGTCTCCCGGGGACAGAGCCACCCTCTCCTGCAGGGCCAGTGAGAGTGTCAACAG24490.17836061180093238No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23950.1744277930842111No Hit
GTATGGAAGTACATTCTGGTCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT23810.173408173416913No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC23630.1720972338446726No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23320.16983950458136965No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23130.16845573503289366No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22470.16364895660134546No Hit
GTCCCAGACCCACTACCACTGAACCTGTCTGGGATGCCACTGGCCCTACT22130.1611727374093358No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATT21820.15891500814603285No Hit
CTCCAGGCACCCTGTCTATGTCTCCCGGGGACAGAGCCACCCTCTCCTGC20620.15017541099776338No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGAC20190.1470437220196335No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT20090.14631542225727773No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAC19600.14274675342173435No Hit
CTTCCATACAGCTGACAGTAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC19490.14194562368314298No Hit
GAGTGAAGTCTGTCCCAGACCCACTACCACTGAACCTGTCTGGGATGCCA19120.13925091456242658No Hit
TCTCTGGTGCATCCAGTAGGGCCAGTGGCATCCCAGACAGGTTCAGTGGTAGTGGGTCTGGGACAGACTTCACT19110.139178084586191No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18940.13793997499018618No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18880.1375029951327727No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTGAGAGTGTCAACAGCAGGTACTTA18300.13327885651110913No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATTTTGCAGTGTA18250.13291470662993124No Hit
GTGGTAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATTTTGCAGTGTATTA17930.1305841473903927No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17580.12803509822214745No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17220.12541321907766662No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17060.12424793945789736No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCACCGGAGAAATTGTGTTGACGCAGTCTC16820.12250002002824346No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16800.1223543600757723No Hit
GACATAGACAGGGTGCCTGGAGACTGCGTCAACACAATTTCTCCGGTGGT16270.11849437133528663No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15930.11601815214327695No Hit
GCCTGGTACCATCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCATCCAGTAGGGCCAGTGGCATC15850.11543551233339233No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15780.11492570249974328No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15610.11368759290373845No Hit
GTACTTAGCCTGGTACCATCAGAGACCTGGCCAGGCTCCCAGGCTCCTCA15570.11339627299879612No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15490.1128136331889115No Hit
CCTCATCTCTGGTGCATCCAGTAGGGCCAGTGGCATCCCAGACAGGTTCAGTGGTAGTGGGTCTGGGACAGACTT15310.11150269361667106No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14890.10844383461477676No Hit
TCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCATCCAGTAGGGCCAGTGGCATCCCAGACAGGTT14370.10465667585052667No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14300.10414686601687763No Hit
CTGCTACTCTGGCTCCCAGAAACCACCGGAGAAATTGTGTTGACGCAGTC14300.10414686601687763No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14250.10378271613569973No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13980.10181630677733909No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13810.10057819718133426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC5700.044.1297141
GGTATCA11700.043.3456761
TCGTATG5550.042.78179640
ATGCCGT5800.041.83825744
GTGGTAT2950.041.2592051
CGCGTAT503.9528604E-641.2310439
GCCGTCT5300.040.7215246
ATCTCGT5300.040.70942337
GATCTCG5500.040.41031636
GTATGCC6600.039.22902342
CGTGGTT350.001397439239.14012537
ACGAGAC8000.037.6159522
CCGTCGT654.9835035E-737.58250847
CTTATAC8150.037.3357851
CCGTCTT5800.037.30480647
CGAGACA8200.037.11894623
GGATCTC6200.036.89920835
TATACAC8350.036.4229353
TCTCCGA8650.035.5516711
TACACAT8750.035.5302855
Produced by FastQC (version 0.11.5)