FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065996
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTATCCGTGGACCTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT76530.7179201422894645No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGACAGTGAATTCTGTC71930.6747680103865306No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39790.37326594096037885No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAAGCAGGAAAAGCCC34490.3235471802896071No Hit
GTAATACACAGCCGTGTCCTCGTCTGTCAGGCTGTTCATTTGCAGATACA32300.3030030131445146No Hit
GTGTATTACTGTGCGAGAGATTTCGGCGTGCAGTGGCTGGCTTCCGCGTGGGGCCAGGGAACCCTGGTCACCGT28470.26707417288620217No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27980.2624775327487157No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27470.257693274646434No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27090.25412853331532204No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24770.23236484939905966No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA22800.2138844798667162No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22500.2110702103947857No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20640.19362173966881677No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20080.18836843665454656No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC19000.17823706655559685No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18170.17045092101658918No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17860.167542842562261No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17830.167261415615068No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17070.16013193295284409No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16910.15863098923448116No Hit
CCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCG16490.15469101197377852No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16330.1531900682554156No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15720.14746772032915695No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14890.1396815747901493No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14820.13902491191336552No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14210.13330256398710688No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14170.13292732805751617No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14140.1326459011103231No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAAGGTC14090.13217685619833472No Hit
GAATTCACTGTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAATAGTTAT14010.13142638433915324No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13760.12908115977921117No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTGCCTTTAGCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG13720.12870592384962046No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13580.12739259809605288No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAAGCAGGAAAAGCCCCT13520.1268297442016668No Hit
CAGTATAATAGTTATCCGTGGACCTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT13470.12636069928967839No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13380.12551641844809924No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13290.1246721376065201No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAACTGGTGGAGT13220.12401547472973631No Hit
CAGTAATACACAGCCGTGTCCTCGTCTGTCAGGCTGTTCATTTGCAGATA13050.1224207220289757No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12960.12157644118739658No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12890.1209197783106128No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGA12860.12063835136341976No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12560.11782408189148928No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAG12560.11782408189148928No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12490.1171674190147055No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGAGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC12430.11660456512031939No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGAGCCAAATGTGACAT12410.11641694715552404No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12230.11472838547236576No Hit
CATCTACAAGGCGTCAAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA11890.11153888007084455No Hit
GTATTACTGTGCGAGAGATTTCGGCGTGCAGTGGCTGGCTTCCGCGTGGGGCCAGGGAACCCTGGTCACCGTCTC11870.11135126210604918No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11790.11060079024686773No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11790.11060079024686773No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11750.11022555431727699No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11720.10994412737008394No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC11690.10966270042289089No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11510.10797413873973259No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11500.10788032975733494No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11270.10572272316218823No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10740.10075084709511103No Hit
CAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA10740.10075084709511103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG651.19162E-842.24139835
GTGGTAT2750.040.910831
TAACGGC702.2782842E-839.2501236
ACCCGGT750.001514584438.49952770
TCCTATA451.0735227E-437.8839532
TCTGGAG12100.035.79501370
GTATCAA19350.032.7709471
GCTTGTA851.3411591E-732.0869261
ATTCTGT21000.031.89466568
TTCTGTC24250.031.18185869
TAAGCTC450.00433214231.07652
GTATAAA902.2140011E-730.3043171
GAATTCT20900.029.9867366
GTATAGG803.0294123E-629.8308121
TGAATTC20850.029.78509965
AATTCTG21300.029.77081567
ATCAGGC20100.029.73050136
GAATACG704.17868E-529.2192789
ATACGTA704.1826956E-529.21516411
ATCAACG22100.029.1559093
Produced by FastQC (version 0.11.5)