FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621257
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57220.35293602433173765No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54810.33807101526778294No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46210.28502575470761266No Hit
GGCTAACAGTTTCCCCGTCACCTTCGGCCAAGGGACACGACTGGAGATTAAAGGAACTGTGGCTGCACCAT41630.2567760694325452No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40950.2525817930161597No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38230.23580468735061744No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC38170.23543460413740697No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32850.2026205592327435No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31580.1947871312197881No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30980.19108629908768318No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30820.19009941051912188No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC30730.18954428569930615No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGAAATTGGTTAGCCTGGTATCAGCAGAAA30700.1893592440927009No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28920.17838010876745636No Hit
GTAATAAACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA27620.17036163914789573No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27590.1701765975412905No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27400.16900466736612393No Hit
ATTCTGGATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA27170.16758601504881707No Hit
GGGTATTAGAAATTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATTCTGGATC26870.16573559898276463No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC25270.15586671329715152No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24670.15216588116504662No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG24660.15210420062951155No Hit
GATCTATTCTGGATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA24010.14809496581973122No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23920.1475398409999155No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTTC23820.1469230356445647No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23310.1437773283322755No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22700.14001481566463553No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22690.13995313512910043No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22520.13890456602500406No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGACGGGGAAA22380.13804103852751293No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21620.1333533178268467No Hit
GGACACGACTGGAGATTAAAGGAACTGTGGCTGCACCATCTGTCTTCATC21520.1327365124714959No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA21490.13255147086489064No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT20980.12940576355260147No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20430.12601333409817198No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20390.12576661195603164No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19660.1212639328619707No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19450.11996864161573396No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18920.11669957323237462No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18770.1157743651993484No Hit
GTGCAGCCACAGTTCCTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG18410.11355386592008547No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18390.1134305048490153No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT18340.11312210217133989No Hit
GTCGTGTCCCTTGGCCGAAGGTGACGGGGAAACTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGG17980.11090160289207694No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17970.11083992235654187No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17900.11040815860779629No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATCCAGAATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG17710.10923622843262974No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17680.1090511868260245No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17670.10898950629048942No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17660.10892782575495433No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17560.10831102039960351No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17480.10781757611532286No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT17440.10757085397318253No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC17130.10565875737159501No Hit
CTAATACCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCTACAGA17090.10541203522945468No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17080.10535035469391958No Hit
ATCCAGAATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACCAATTTCTAATACC16800.1036232996989373No Hit
GAATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC16250.10023087024450782No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16220.10004582863790257No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG200.007670352251.211435
GTGGTAT4150.042.3076551
TACGTAT1354.5833804E-737.9822970
TCTGGAG14200.036.1099270
GTATCAA35400.033.669381
ATCAACG37200.031.4058273
TCAACGC37250.031.3607674
TATCAAC37950.030.9631082
AACGCAG38200.030.9334356
CAACGCA38150.030.8854795
ACGCAGA40700.029.11637
CGCAGAG41250.028.6462358
GACAGGT3550.028.53361
AGAGTAC41600.028.316211
GACCGCG606.1548245E-428.1346977
GCAGAGT42050.028.1012429
GCCGTGT10850.027.99726711
GACAGCC2900.027.943251
AGCTCTC10800.027.87111522
CAGAGTA42750.027.79733810
Produced by FastQC (version 0.11.5)