FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812151
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT53690.2962777384445336No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA48840.2695139643440309No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC45700.2521864899779323No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG42810.23623859159639568No Hit
GTCTCTGGTGCCTCCGTCAGCAGTAATTATTATTACTGGGGCTGGATCCG42750.23590749336010078No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG40760.22492606852298733No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGTTCCCTTCATGCGTGAC40310.22244283175077573No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA38150.2105232952441601No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC37840.20881262102330325No Hit
CTATTATTGTGTGAGCCAGGCTTACGATCTCTGGACTGGTTATCACACGCCTCCTGTGGACTGGGGCCAGGGAAC36450.20114217854913857No Hit
GTGTTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG34740.19170587881473453No Hit
GCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATT34600.1909333162633798No Hit
CACCTGCACTGTCTCTGGTGCCTCCGTCAGCAGTAATTATTATTACTGGG34580.19082295018461487No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT34300.18927782508190544No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC34300.18927782508190544No Hit
CAATAATAGACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCCTCAGGGA34030.1877878830185785No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG32120.17724792249652485No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGTAGTGACGTT29620.16345216265090493No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC29340.16190703754819546No Hit
ATAATAGACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCCTCAGGGAGA29250.16141039019375317No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC29090.16052746156363348No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGTTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28940.1596997159728963No Hit
GTCTATTATTGTGTGAGCCAGGCTTACGATCTCTGGACTGGTTATCACAC28770.15876160430339414No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC28760.15870642126401166No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT27060.1493253045689901No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG26830.14805609466319308No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT26290.14507621053653919No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG25680.1417100451342079No Hit
CCTGTGGACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCG25380.1400545539527335No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC25330.13977863875582114No Hit
GAGTTATGATTTATGAGGTCAATAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACA25240.1392819914013788No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC25100.1385094288500241No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGTAGTGACGTTGGTGGTTATAGCTATGTCTCCTGGTACCAACAATA25070.13834387973187665No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATTGTTGCTGC24980.13784723237743432No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTATTGCAGCTCATATGCAGGCAGCAACAATGTCTTCGGAA24890.13735058502299202No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCACGTGCGAGAAAATGAAGCGCCTGTGGTT24860.13718503590484457No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG24840.1370746698260796No Hit
GTCTTCTCCACGGTGTTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24780.13674357158978473No Hit
CTCCTGCACTGGAACCAGTAGTGACGTTGGTGGTTATAGCTATGTCTCCTGGTACCAACAATACCCAGGCAAGGC24410.134701799132633No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC24270.13392923658127828No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA24030.13260484363609876No Hit
GGTTATCACACGCCTCCTGTGGACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCCTCAGCATCCCCGACCAGC23970.13227374539980388No Hit
GGACTGGAGTGGATTGGAAGTATCTTTTATAGTGGGAGCTCCTACTACAA23760.1311149015727718No Hit
CCCTGAGGCTGACCTCTGTGACCGCCGCAGACACGGCTGTCTATTATTGTGTGAGCCAGGCTTACGATCTCTGGA23750.13105971853338932No Hit
ACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATTGTTGCTGCCTGCATATGAGCTGCAATAATAATCAGC23660.13056307117894703No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG23470.1295145934306799No Hit
GGATGAGGCTGATTATTATTGCAGCTCATATGCAGGCAGCAACAATGTCTTCGGAACTGGGACCAAGGTCACCGT23020.12703135665846832No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG22080.12184415095651523No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT21210.11704322653023948No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC20900.11533255230938261No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20180.11135937347384406No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20120.1110282752375492No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT20080.11080754308001926No Hit
CTTTTATAGTGGGAGCTCCTACTACAACCCGGCCCACAAGAGTCGAGTCACCATATCCGTGGACACGGCCAAGAA20040.11058681092248936No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC20010.11042126180434192No Hit
GTCCACAGGAGGCGTGTGATAACCAGTCCAGAGATCGTAAGCCTGGCTCA19700.10871058758348505No Hit
GTCCCTCACCTGCACTGTCTCTGGTGCCTCCGTCAGCAGTAATTATTATT19690.10865540454410257No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT19580.10804839111089527No Hit
GACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATTGTTGCTGCCTGCATATGAGCTGCAATAATAATCAG19330.10666881512633329No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC19170.10578588649621362No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG19100.10539960522053626No Hit
CCCCTGAGGGCCGCTTATTGACCTCATAAATCATAACTCTGGGGGCCTTGCCTGGGTATTGTTGGTACCAGGAGA18850.10402002923597425No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18770.10357856492091444No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA18570.10247490413326484No Hit
GCCTACCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTA18480.10197825677882252No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT18460.10186789070005756No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT18220.10054349775487803No Hit
GTCATGGATCTCACGTGCGAGAAAATGAAGCGCCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGT18130.10004685040043572No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)