FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812151
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT48050.26515450423281506No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA46440.25627003489223577No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGTTCCCTTCATGCGTGAC46080.2542834454744665No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG41320.2280163187284062No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC41180.22724375617705148No Hit
CAATAATAGACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCCTCAGGGA40310.22244283175077573No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG39260.21664861261561535No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38130.21041292916539517No Hit
GTGTTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG37730.20820560759009596No Hit
GTCTCTGGTGCCTCCGTCAGCAGTAATTATTATTACTGGGGCTGGATCCG37540.20715712984182888No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37350.20610865209356172No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT35700.1970034505954526No Hit
ATAATAGACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCCTCAGGGAGA35080.19358210215373886No Hit
CTATTATTGTGTGAGCCAGGCTTACGATCTCTGGACTGGTTATCACACGCCTCCTGTGGACTGGGGCCAGGGAAC34650.1912092314602922No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA33390.18425616849809975No Hit
GCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATT32280.17813085112664453No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC31580.17426803836987095No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC31450.17355065885789872No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC31130.17178480159765935No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC29490.1627347831389327No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGTAGTGACGTT29180.1610241089180758No Hit
CACCTGCACTGTCTCTGGTGCCTCCGTCAGCAGTAATTATTATTACTGGG28820.15903751950030653No Hit
GAGTTATGATTTATGAGGTCAATAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACA28320.15627836753118257No Hit
GTCTATTATTGTGTGAGCCAGGCTTACGATCTCTGGACTGGTTATCACAC28040.15473324242847314No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC27920.15407104595588336No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG27560.1520844565381141No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGTTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26910.14849755897825292No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG26810.14794572858442812No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG26470.1460695052454238No Hit
GTCTTCTCCACGGTGTTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26340.14535212573345158No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCACGTGCGAGAAAATGAAGCGCCTGTGGTT26190.14452438014271438No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATTGTTGCTGC26190.14452438014271438No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT26010.14353108543382972No Hit
CTCCTGCACTGGAACCAGTAGTGACGTTGGTGGTTATAGCTATGTCTCCTGGTACCAACAATACCCAGGCAAGGC25800.14237224160679765No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA25340.1398338217952036No Hit
CCTGTGGACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCG25150.1387853440469365No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC24710.13635729031410737No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG23520.12979050862759228No Hit
GGATGAGGCTGATTATTATTGCAGCTCATATGCAGGCAGCAACAATGTCTTCGGAACTGGGACCAAGGTCACCGT23320.1286868478399427No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTATTGCAGCTCATATGCAGGCAGCAACAATGTCTTCGGAA23260.12835574960364782No Hit
CCCTGAGGCTGACCTCTGTGACCGCCGCAGACACGGCTGTCTATTATTGTGTGAGCCAGGCTTACGATCTCTGGA23160.12780391920982304No Hit
GGTTATCACACGCCTCCTGTGGACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCCTCAGCATCCCCGACCAGC23120.1275831870522931No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGTAGTGACGTTGGTGGTTATAGCTATGTCTCCTGGTACCAACAATA23000.12692099057970335No Hit
GTCCACAGGAGGCGTGTGATAACCAGTCCAGAGATCGTAAGCCTGGCTCA22950.12664507538279096No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT22600.12471366900440417No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGGAGACGGTGACCAGGGTTCCC22200.12250634742910498No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT21340.11776060604221171No Hit
ACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATTGTTGCTGCCTGCATATGAGCTGCAATAATAATCAGC21150.1167121282939446No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC20910.11538773534876508No Hit
GTGATAACCAGTCCAGAGATCGTAAGCCTGGCTCACACAATAATAGACAGCCGTGTCTGCGGCGGTCACAGAGGT20220.111580105631374No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA20000.11036607876495942No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG19760.10904168581977991No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC19590.10810357415027777No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG19110.10545478825991873No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18830.1039096631572093No Hit
CCCCTGAGGGCCGCTTATTGACCTCATAAATCATAACTCTGGGGGCCTTGCCTGGGTATTGTTGGTACCAGGAGA18660.10297155148770716No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG18630.10280600236955971No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT18570.10247490413326484No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGGAGACGGTGACCAGGGTTCC18560.10241972109388237No Hit
GGGTTCCCTGGCCCCAGTCCACAGGAGGCGTGTGATAACCAGTCCAGAGA18420.10164715854252764No Hit
GACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATTGTTGCTGCCTGCATATGAGCTGCAATAATAATCAG18220.10054349775487803No Hit
GGTTATAGCTATGTCTCCTGGTACCAACAATACCCAGGCAAGGCCCCCAGAGTTATGATTTATGAGGTCAATAAG18190.10037794863673061No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTAT200.00686376952.6710957
GGTATCA14350.043.8221131
GTGGTAT3400.040.207631
GTATCAA35550.038.6468241
CGTCTAG400.00239542335.0722756
TATCAAC39900.034.3449482
TGGTATC3850.033.729842
ATCAACG41000.033.2512863
TCAACGC41250.032.882294
CAACGCA41950.032.334495
AACGCAG42400.032.07196
CTGGAGT22650.031.92794470
ACGCAGA45050.030.1853167
CGCAGAG45250.030.1274098
GAGTTTA5600.029.9044231
AGAGTAC46350.029.4804311
GCAGAGT46350.029.41169
CAGAGTA46750.029.37757310
GACAGGT3600.028.4804021
AGTTTAT5800.028.2816452
Produced by FastQC (version 0.11.5)