FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403979
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT35150.870094732646994No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10820.2678357043311657No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10550.2611521886038631No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10420.2579341995499766No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8440.20892175088309045No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8410.2081791380245013No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC7930.19629733228707433No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG7180.17773201082234474No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG6950.17203864557316098No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT6850.1695632693778637No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG6800.16832558128021508No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT6550.16213714079197186No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC6270.15520608744513947No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC6240.1544634745865503No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT5850.1448095074248909No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC5820.1440668945663017No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA5800.14357181932724225No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA5500.1361456907413504No Hit
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG5270.13045232549216668No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC4940.12228358404768565No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG4660.11535253070085326No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT4540.11238207926649653No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT4510.11163946640790734No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA4500.11139192878837761No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTC4470.11064931592978842No Hit
GCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGAC4470.11064931592978842No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG4440.10990670307119924No Hit
GAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGG4300.10644117639778305No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG4190.10371826258295605No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC4170.10322318734389659No Hit
GGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAA4150.10272811210483714No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG4100.1014904240071885No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGAT4060.10050027352906958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG5700.079.6984270
GTAACGG150.002006254671.8400550
GGCGGTA200.00860560849.73542425
GCGATAT200.00867757349.63074515
CTCTTAT1950.047.533311
ATCGTCA352.904712E-547.27322816
GGTATCA7550.046.4765131
AATATCG405.50228E-542.4252336
TTCTGCG350.001058399241.40765452
AAAGGGG6300.040.331768
TATACAC8700.039.9228973
CTTATAC8700.039.1915631
TTATACA8700.038.782242
GTATCAA19650.038.7471661
TATCGTA451.0569715E-437.9765838
CGTATGC8850.037.92730741
ATATCGT451.0802685E-437.8362537
GCCGTCT8400.037.82982346
GTATGCC9100.037.7975942
CGTCTTC8200.037.7550548
Produced by FastQC (version 0.11.5)