FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583170
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA21510.36884613406039407No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19660.3371229658590119No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17500.3000840235265874No Hit
GGCTAACAGTTTCCCCGTCACCTTCGGCCAAGGGACACGACTGGAGATTAAAGGAACTGTGGCTGCACCAT15890.2724762933621414No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13740.23560882761458923No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13710.23509439785997224No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13690.23475144469022755No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13040.2236054666735257No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGAAATTGGTTAGCCTGGTATCAGCAGAAA11040.1893101496990586No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC11000.18862424335956926No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC10610.18193665654954816No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10340.17730678875799508No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10330.17713531217312276No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10260.1759349760790164No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT10080.17284839755131437No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10000.1714765848723357No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9980.171133631702591No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9930.17027624877822933No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9590.16444604489256992No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9260.15878731759178283No Hit
GATCTATTCTGGATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA8700.14918462883893205No Hit
ATTCTGGATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA8680.14884167566918738No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8530.14626952689610234No Hit
GGGTATTAGAAATTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATTCTGGATC8270.1418111356894216No Hit
GGACACGACTGGAGATTAAAGGAACTGTGGCTGCACCATCTGTCTTCATC8010.1373527444827409No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7860.13478059570965586No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7830.13426616595503885No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7750.13289435327606014No Hit
GTTTATTACTGTGCGAAAGATGTGCGGGTGGTAGCTGCTCGCTGGTTCGA7680.1316940171819538No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT7560.12963629816348576No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7400.1268926728055284No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7360.12620676646603907No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTTC7270.12466347720218804No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7260.1244920006173157No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTACATGGCATAGCTAC7140.12243428159884767No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7010.12020508599550732No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT6920.11866179673165628No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGACGGGGAAA6900.11831884356191162No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATGCCATGTACTGGGTCCGCCAGGCTCCAGGCAAGGGG6830.11711850746780528No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6830.11711850746780528No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT6760.11591817137369892No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6700.1148893118644649No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG6700.1148893118644649No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6660.11420340552497556No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6490.11128830358214585No Hit
GAATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC6480.11111682699727352No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG6390.10957353773342252No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6290.10785877188469915No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6240.10700138896033745No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6170.10580105286623112No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC6170.10580105286623112No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6160.10562957628135877No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6120.10494366994186943No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6050.10374333384776309No Hit
CTAATACCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCTACAGA5980.10254299775365673No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT5950.10202856799903973No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC5940.10185709141416739No Hit
GTCTCCTACAGATGCAGACACGGAAGATGGAGACTGGGTCATCTGGATGTCGCATCTGGAACCTGGGAACCAGAG5940.10185709141416739No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC5840.10014232556544403No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAAT100.0071790796144.3824370
CGTATGG150.002374394668.8569841
GTCTTAA209.465665E-567.5337141
TGCGTGA352.6191265E-761.11038255
ATCTCGT2350.056.74944337
GATCTCG2350.056.6903436
GCGATCT2500.055.8133934
CGATCTC2450.055.6451535
ATGCCGT3450.055.18043544
TCGTATG3700.054.7940440
CGTATGC3650.054.70828241
CGTGAAA406.349146E-753.75885457
CCGTCTT3400.053.37907447
GTATGCC3750.052.40923742
CGAGACA4450.052.3620923
GAGCGAT4050.052.00927732
CTTATAC4550.051.9490131
ACGAGAC4500.051.77140822
TATACAC4500.051.7447743
GTACAGA200.00800924250.6502881
Produced by FastQC (version 0.11.5)