FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449049
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24700.5500513307011039No Hit
GTATTACTGTCAGCAATATTATGCTAAGCCGCTCACCTTCGGCGGAGGGA24000.5344628314504652No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23990.5342401386040276No Hit
GTTTTATACTACTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA23850.5311224387538999No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17160.3821409244870827No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15370.3422789049747355No Hit
GTGTATTACTGTCAGCAATATTATGCTAAGCCGCTCACCTTCGGCGGAGGGACCAAGGTGGAAATCAAACGAA15070.3355981195816047No Hit
ATATTATGCTAAGCCGCTCACCTTCGGCGGAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT14340.319341541791653No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14050.3128834492449599No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13040.29039147175475283No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12700.2828199149758712No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12580.2801476008186189No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12580.2801476008186189No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12110.2696810370360473No Hit
GCTCATGTACTGGGCATCTACCCGGGGATCCGGGGTCCCTGACCGATTCACCGGCAGCGGGTCTGTGACGGATTT12030.26789949426454573No Hit
GTACATGAGCAGTTGAGGAGGCTGTCCCGGTTTCTGCTGGTACCAAGCTA11910.2652271801072934No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11820.2632229444893541No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11340.25253368786034486No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10940.24362597400283711No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10800.2405082741527094No Hit
CTCCTCAACTGCTCATGTACTGGGCATCTACCCGGGGATCCGGGGTCCCTGACCGATTCACCGGCAGCGGGT9380.20888588995855686No Hit
GTATAAAACACTCTGGCTGGACTTGCAGCTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGATTCTGGAGA9380.20888588995855686No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9240.20576819010842914No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8950.199310097561736No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8930.19886471186886062No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8710.19396546924723138No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8330.185503141082599No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8280.18438967685041055No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8040.1790450485359059No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7790.17347772737496353No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7690.17125079891058662No Hit
GTTGAGGAGGCTGTCCCGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGTAGTATAAAACACT7480.166574249135395No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7130.15877999951007574No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7100.15811192097076265No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7060.15722114958501188No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6900.15365806404200877No Hit
ATGCTAAGCCGCTCACCTTCGGCGGAGGGACCAAGGTGGAAATCAAACGA6700.14920420711325488No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6610.14719997149531566No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6580.14653189295600258No Hit
CAGTAATACACTGCCACATCTTCCGCCTGCAGGCTGCTGATGGTGAGAGT6580.14653189295600258No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT6560.1460865072631272No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6550.1458638144166895No Hit
GTCCCTGACCGATTCACCGGCAGCGGGTCTGTGACGGATTTCACTCTCAC6510.14497304303093872No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGCGC6490.14452765733806333No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT6390.1423007288736864No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6340.14118726464149792No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6250.13918302902355867No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6230.1387376433306833No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG6230.1387376433306833No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC5900.1313887793982394No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG5870.13072070085892631No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC5870.13072070085892631No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC5870.13072070085892631No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA5820.12960723662673784No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC5770.12849377239454937No Hit
CCTTCGGCGGAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCA5770.12849377239454937No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC5690.12671222962304782No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA5630.12537607254442165No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA5630.12537607254442165No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA5620.12515337969798396No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT5400.1202541370763547No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC5380.11980875138347931No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC5300.11802720861197775No Hit
GTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCAGAGTGTTTTATACTACTCCAACAATAAGAACTA5230.1164683586869139No Hit
CCAGAATCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCAGAGTGTTTTATACTA5150.11468681591541235No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA5140.11446412306897465No Hit
CTCATGTACTGGGCATCTACCCGGGGATCCGGGGTCCCTGACCGATTCAC5060.1126825802974731No Hit
GTGCTGACCCAGTCTCCAGAATCCCTGGCTGTGTCTCTGGGCGAGAGGGC5050.11245988745103543No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTCCGCCGAAGGTG5010.11156911606528463No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC5010.11156911606528463No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC4940.11001026614022077No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC4890.1088968019080323No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT4890.1088968019080323No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG4860.10822872336871923No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA4640.10332948074708995No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG4640.10332948074708995No Hit
CACCATCAGCAGCCTGCAGGCGGAAGATGTGGCAGTGTATTACTGTCAGCAATATTATGCTAAGCCGCTCACCTT4560.10154793797558842No Hit
GCATAATATTGCTGACAGTAATACACTGCCACATCTTCCGCCTGCAGGCT4530.10087985943627532No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA200.007041877652.32428747
CTAACGC200.00803251250.611223
CGTATGC1200.048.8660541
ATATAAC250.00977915848.15136768
GGTATCA3800.047.9581531
ACTAGAT1000.047.61359432
TCGTATG1250.046.85806740
ACCGTAC1100.046.12319627
CCGTCTT1300.045.61604747
GCCGTCT1250.044.5628446
ATCTCGT1000.044.4772237
TACTAGA1150.044.30578631
CGTACTA1150.044.2066729
ATCTCCG1300.044.12751810
GACCGTA1150.044.0981526
ATGCCGT1300.042.6528544
TCTCCGA1350.042.4931611
CGTCTTC1400.042.43102648
GATCTCG1050.042.30193736
CTTATAC1400.040.9801141
Produced by FastQC (version 0.11.5)