FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449049
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23890.5320132101396506No Hit
GTATTACTGTCAGCAATATTATGCTAAGCCGCTCACCTTCGGCGGAGGGA23220.5170927894283253No Hit
CAGTAATACACTGCCACATCTTCCGCCTGCAGGCTGCTGATGGTGAGAGT19750.4398183717144454No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17880.3981748094305967No Hit
GTTTTATACTACTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA16180.3603170255361887No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15940.35497239722168406No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15790.3516320045251186No Hit
GTGTATTACTGTCAGCAATATTATGCTAAGCCGCTCACCTTCGGCGGAGGGACCAAGGTGGAAATCAAACGAA15480.3447285262855501No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15130.33693427666023085No Hit
ATATTATGCTAAGCCGCTCACCTTCGGCGGAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT14560.3242407844132823No Hit
GTAATACACTGCCACATCTTCCGCCTGCAGGCTGCTGATGGTGAGAGTGA12030.26789949426454573No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11840.2636683301822295No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11670.25988255179278874No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11490.25587408055691024No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11410.25409253778540875No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11290.2514202236281564No Hit
GTACATGAGCAGTTGAGGAGGCTGTCCCGGTTTCTGCTGGTACCAAGCTA11200.24941598801021714No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10630.2367224957632686No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10570.23538633868464243No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10400.23160056029520162No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10390.23137786744876396No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10140.2258105462878216No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9730.21668013958387614No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9590.21356243973374842No Hit
GCTCATGTACTGGGCATCTACCCGGGGATCCGGGGTCCCTGACCGATTCACCGGCAGCGGGTCTGTGACGGATTT9200.20487741872267837No Hit
GCATAATATTGCTGACAGTAATACACTGCCACATCTTCCGCCTGCAGGCT8880.19775124763667215No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8650.1926293121686052No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8410.18728468385410055No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7920.17637273437865356No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7820.1741458059142766No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7780.17325503452852584No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7210.1605615422815773No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7060.15722114958501188No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6860.152767292656258No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6840.15232190696338263No Hit
GTTGAGGAGGCTGTCCCGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGTAGTATAAAACACT6790.15120844273119413No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG6690.1489815142668172No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6600.14697727864887794No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC6590.14675458580244027No Hit
ATGCTAAGCCGCTCACCTTCGGCGGAGGGACCAAGGTGGAAATCAAACGA6480.14430496449162564No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6460.14385957879875025No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6380.1420780360272487No Hit
GTATAAAACACTCTGGCTGGACTTGCAGCTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGATTCTGGAGA6310.14051918610218483No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6210.1382922576378079No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTCCGCCGAAGGTG6160.13717879340561942No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6160.13717879340561942No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA5940.13227955078399017No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC5930.13205685793755248No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC5910.13161147224467706No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC5850.13027531516605093No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA5850.13027531516605093No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5750.12804838670167398No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC5700.1269349224694855No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT5600.12470799400510857No Hit
CTCCTCAACTGCTCATGTACTGGGCATCTACCCGGGGATCCGGGGTCCCTGACCGATTCACCGGCAGCGGGT5510.12270375838716932No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGCGC5430.12092221561566777No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG5340.11891797999772855No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA5240.1166910515333516No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5190.11557758730116313No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC5170.11513220160828774No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT5150.11468681591541235No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC5150.11468681591541235No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA5090.11335065883678619No Hit
GCTTAGCATAATATTGCTGACAGTAATACACTGCCACATCTTCCGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT5020.11179180891172233No Hit
GTATAGGGGGTATCCAGGCGCTACTCTACCGACGACCCTTACACAATAAT5010.11156911606528463No Hit
CCTTCGGCGGAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCA5000.11134642321884694No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC4940.11001026614022077No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT4910.10934218760090769No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT4870.10845141621515693No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC4730.1053337163650292No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG4710.10488833067215382No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC4690.10444294497927843No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG4670.10399755928640304No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA4620.10288409505421457No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC4520.10065716658983763No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTG100.007196398144.2582270
TCGGAGC200.005385914455.9926366
CGCGTAA200.00787539250.8647330
GTCGCGT200.00794894150.74527728
GCTTGTA200.0080336250.6094441
ATGCCGC200.00806199550.56432710
TAGTCTC200.00806199550.5643275
CAACGGA352.4714727E-548.58419432
GGTATCA4800.041.4716261
TAATCTA350.001109863641.01128858
TAGTTCG350.00112353340.90937457
TATTCTA750.001518502838.4688670
CCAACGG451.0981006E-437.73256331
TTTGACT608.517654E-636.85993665
CGGGATC558.59743E-636.81097417
GTATCAA11300.036.1282731
TTGAGTA400.002454870834.8915247
AACGGAA502.0123369E-434.0395533
TTGACTG552.0487396E-433.9349366
TTTATGG502.1183568E-433.743392
Produced by FastQC (version 0.11.5)