FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1273468
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62070.48740918499718877No Hit
CTACTATAGTGTTGGCAGAAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC60500.47508064592121674No Hit
CTATAGTAGTTATTCCCCCTCGGCCACTTTTGGCCAGGGGACCAAGCTGG54810.4303995074866428No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51630.40542832642830445No Hit
GTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT50290.39490587906409896No Hit
GAGTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGTC46940.3685997606535853No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43270.33978081899191814No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40770.3201493873422811No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG40580.31865739853690866No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38480.30216699595121355No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC37170.2918801257668037No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34350.26973587086601314No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31980.2511252736621572No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG30170.23691211714781998No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA29630.23267172791149837No Hit
ACCTTGATGTGACCCCACTTTCTAAACTGGACGCCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTG29440.23117973910612594No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29050.22811723576878257No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA28780.22599704115062175No Hit
GGCCAAAAGTGGCCGAGGGGGAATAACTACTATAGTGTTGGCAGAAATAAGTTGCAAAATCATCAGGCTGCAGG27820.21845857139716113No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27790.2182229942173655No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG27720.21767331413117566No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAA27630.21696658259178875No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27590.21665247968539453No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA27230.21382555352784677No Hit
TCTATAAGGCGTCCAGTTTAGAAAGTGGGGTCACATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT26980.21186241036288306No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26950.21162683318308745No Hit
GTACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA26700.20966369001812374No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26180.20558035223499924No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26120.20510919787540793No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT25530.20047618000609357No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25240.1981989339347357No Hit
CCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTAATACTCTGA25120.1972566252155531No Hit
GTTTAGAAAGTGGGGTCACATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC25100.19709957376235604No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24210.19011078409508522No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG24130.18948257828229684No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23810.1869697550311433No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23230.1824152628884275No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA22000.17275659851680608No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT21960.1724424956104119No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC21220.16663159184211931No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21190.16639601466232368No Hit
ACTATAGTGTTGGCAGAAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC21010.1649825515835498No Hit
CACTATAGTAGTTATTCCCCCTCGGCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCA20970.1646684486771556No Hit
ATAGTAGTTATTCCCCCTCGGCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT20500.16097773952702385No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20410.1602710079876369No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20100.15783671046308193No Hit
GATCTATAAGGCGTCCAGTTTAGAAAGTGGGGTCACATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA19770.1552453614853298No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC19700.15469568139913997No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19230.15100497224900822No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19150.15037676643621983No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18700.14684310873928516No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18600.1460578514732997No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18370.14425175976153307No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17950.14095367924439403No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGTGACCCCA17460.1371059186410652No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17110.134357518210116No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAC16930.1329440551313421No Hit
AACTACTATAGTGTTGGCAGAAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATT16650.1307453347865828No Hit
CTTTCTAAACTGGACGCCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGG16580.13019565470039296No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTA16410.12886071734821763No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15610.12257865922033376No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15580.12234308204053812No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15450.12132224759475699No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTTCTGCCAACACTATAGTAGTTATTCCCCCTCGGC15430.1211651961415599No Hit
CTATAGTAGTTATTCCCCCTCGGCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCAC15430.1211651961415599No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15260.11983025878938458No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15040.11810269280421652No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14760.11590397245945716No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14710.11551134382646444No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14670.11519724092007025No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14600.11464756083388039No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14500.11386230356789492No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14420.11323409775510651No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14340.11260589194231814No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14330.1125273662157196No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGTCCAGTTTAGAAAGTGGGGTCACAT14250.1118991604029312No Hit
GCCCCTAACCTCCTGATCTATAAGGCGTCCAGTTTAGAAAGTGGGGTCACATCAAGGTTCAGCGGCAGTGGAT14070.11048569732415735No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13900.10915075997198202No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13890.10907223424538347No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13740.10789434834640524No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC13700.10758024544001105No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13580.10663793672082847No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13570.10655941099422991No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13460.10569562800164589No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13120.10302575329729526No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12810.10059145577274026No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12790.10043440431954317No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12740.10004177568655043No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT352.3000986E-549.18703539
GTGGTAT2500.044.7222821
CGAATAT551.5816477E-743.50168234
CGTATGC3650.042.61066441
GACTAAG5650.040.83268426
GGCTAAA4100.040.76004470
TCGTATG3550.039.84003440
ATGCCGT3950.039.53758244
CGAGACT5700.039.2362423
GCGAATC3950.038.90212633
ACGAGAC5750.038.89352422
GGCGAAT5200.038.7012932
ACTAAGG5900.038.55474527
GTATCAA26600.038.465851
GCCGTCT3750.038.0622846
AGACTAA6050.037.55597725
CGAATCT3550.037.54974434
CTAAGGC6000.037.37426428
CGAATCG557.88332E-637.28715534
CCCACGA6000.037.2641819
Produced by FastQC (version 0.11.5)