FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1328962
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65540.4931668475095601No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46230.3478654769662338No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCG34500.2596011022136073No Hit
ATCATAATGCTGACAGAAATACACTGCAAGATCTTCAGGCTCCAGTCTGC34100.2565912343618553No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28830.21693622541502314No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28520.2146035778299154No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27490.20685316811165405No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26490.19932849848227413No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATC25430.19135234867513143No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23330.17555054245343357No Hit
GTGTATTTCTGTCAGCATTATGATAGCGCACTCACTTTCGGCGGAGGGAC22900.17231493451280022No Hit
GTCTCCAGGCACCCTGTCTCTGTCGCCAGGGGAAAGAGCCACCCTCTCCT22380.16840210630552266No Hit
GTATTTCTGTCAGCATTATGATAGCGCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCACACGAACTGTGG21720.1634358243501319No Hit
GCATTATGATAGCGCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCACACGAACTGTGGCTGCACCAT21620.16268335738719392No Hit
GTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGACTCCTCATCTATGATACATCCACCAGGGCCA21190.15944774944656057No Hit
CTCCAGGCACCCTGTCTCTGTCGCCAGGGGAAAGAGCCACCCTCTCCTGC20910.15734084195033415No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGGT20100.15124585955053643No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19630.14770926482472788No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19630.14770926482472788No Hit
CCCTGTCTCTGTCGCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGGGTTTGGAATAAGTACTTAG19590.14740827803955267No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19220.1446241502766821No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18720.14086181546199214No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18220.13709948064730218No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18130.136422260380658No Hit
CTCCCAGACTCCTCATCTATGATACATCCACCAGGGCCACTGGCATCCCA18110.1362717669880704No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATCTTGCAGTGTATTTCTGTCAGCATTATGATAGCGCA17880.13454109297331301No Hit
GAAATACACTGCAAGATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17730.13341239252890602No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17690.13311140574373082No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17480.13153122512156104No Hit
GTCAGAGGGTTTGGAATAAGTACTTAGCCTGGTACCAGCAGAAACCTGGC17020.12806987709204626No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAC17000.12791938369945868No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTGATCTCCACCTTGGTCC16890.1270916700402269No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA16770.1261887096847013No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16700.1256619828106447No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16650.12528574932917572No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16320.12280260835148032No Hit
GTGCAGCCACAGTTCGTGTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG15980.12024422067749116No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT15810.11896502684049658No Hit
GTTCTAGGAAGGCCGTGCTCGTGGATGTGTCCGCGGTTATTGTGACTCTGCCCTGCAACTTCTGTGCGTAGTTTG15800.11888978014420278No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15080.11347201801104921No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14940.11241856426293603No Hit
GTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTACTTATTC14820.11151560390741044No Hit
CTGCTACTCTGGCTCCCAGAAACCGCCGGAGAAATTGTATTGACGCAGTC14730.11083838364076624No Hit
CAGAAATACACTGCAAGATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA14620.11001066998153446No Hit
ATAATATACGGCCGTGTCTTCAGATCTCAGACTGTTCAGTTCTAGGAAGG14540.10940869641118407No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCGCCGGAGAAATTGTATTGACGCAGTCTC14520.10925820301859648No Hit
CTCCTGCAGGGCCAGTCAGAGGGTTTGGAATAAGTACTTAGCCTGGTACC14100.1060978417742569No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT14010.10542062150761271No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCTTGCAAGGCTTCTGGAGGCTCCTTCAGCAC13910.10466815454467471No Hit
GAATAAGTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGAC13870.10436716775949953No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13820.10399093427803054No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13650.10271174044103593No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13420.10098106642627855No Hit
TCCTAGAACTGAACAGTCTGAGATCTGAAGACACGGCCGTATATTATTGT13390.10075532633739714No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)