FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1411987
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42520.30113591697373987No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41920.2968865860663023No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT37870.268203602441099No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29960.21218325664471416No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC29000.2053843271928141No Hit
GTAATAGACGGCCGTGTCCTCAGATGTCAGGCTGCCCAACTCCAGGTAGGCTGTAGTTATGGAGATGTTCCTGGT28300.20042677446747031No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28000.19830210901375153No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27950.19794799810479843No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26770.1895909806535046No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26650.1887411144720171No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25570.18109231883862953No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25380.17974669738460763No Hit
CTCTTGTAGGACCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTTCCAGCACCGACCTGGCCAGGCTCCCAGGCT23130.1638117064817169No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT22310.1580042875748856No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22210.15729606575697935No Hit
GTCTCCGGCCACCCTGTCTGTGTCTCCGGGGGAAAGCGCCACCCTCTCTTGTAGGACCAGTCAGAGTGTTAGCAG21950.1554546890304231No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCCGCCTGCAGTCTGAAGATT21800.1543923563035637No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21760.1541090675764012No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21350.15120535812298555No Hit
GTCTGTGTCTCCGGGGGAAAGCGCCACCCTCTCTTGTAGGACCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTT20450.14483136176182926No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19790.14015709776364796No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19530.1383157210370917No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACCCCAGTCGCCCTGGA19460.13781996576455732No Hit
ACTCTGGTCCCAGGGCCGAAAGTGAATATCGGAGGCCATCTATCATTCTG19290.13661598867411667No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19100.13527036722009478No Hit
GACTTCACTCTCACCATCAGCCGCCTGCAGTCTGAAGATTTTGCGATTTATTACTGTCAGCAGAATGATAGATGG18810.13321652394816666No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGCGCTCGACTCTGGTCCCAGGGCCGAAAGTGAATATCGGAGG18010.12755074940491662No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17660.12507197304224474No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT17310.12259319667957283No Hit
GAATGATAGATGGCCTCCGATATTCACTTTCGGCCCTGGGACCAGAGTCG17230.12202661922524782No Hit
GGAATATGCACCGTAGATGAGGAGCCTGGGAGCCTGGCCAGGTCGGTGCT17140.1213892195891322No Hit
GTCAGAGTGTTAGCAGCAACTTAGCCTGGTTCCAGCACCGACCTGGCCAGGCTCCCAGGCTCCTCATCTACGGTG16960.12011442031690094No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16950.12004359813511031No Hit
GCATATTCCAGGGCGACTGGGGTCCCAGCCAGGTTCAGTGGCAGTGGGTC16620.11770646613601966No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16360.11586508940946341No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACCCCA16020.11345713522858214No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC15660.11090753668411961No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15490.10970355959367899No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15120.10708313886742583No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15020.10637491704951958No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC14900.10552505086803207No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14880.10538340650445083No Hit
GTGCATATTCCAGGGCGACTGGGGTCCCAGCCAGGTTCAGTGGCAGTGGG14670.10389614068684767No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14560.10311709668715081No Hit
GACCAGAGTCGAGCGCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT14330.10148818650596642No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)