FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1411987
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77240.5470305321507918No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43100.3052436035175961No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35470.2512062788113488No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29540.20920872500950785No Hit
GTAATAGACGGCCGTGTCCTCAGATGTCAGGCTGCCCAACTCCAGGTAGGCTGTAGTTATGGAGATGTTCCTGGT28600.2025514399211891No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28540.2021265068304453No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26510.18774960392694834No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC25910.1835002730195108No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23450.16607801629901692No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23150.16395335084529813No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23130.1638117064817169No Hit
CTCTTGTAGGACCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTTCCAGCACCGACCTGGCCAGGCTCCCAGGCT22830.16168704102799814No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCCGCCTGCAGTCTGAAGATT22690.16069553048292937No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21610.15304673484954182No Hit
GTCTGTGTCTCCGGGGGAAAGCGCCACCCTCTCTTGTAGGACCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTT21530.1524801573952168No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21250.1504971363050793No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21120.14957644794180117No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20770.14709767157912926No Hit
GTCTCCGGCCACCCTGTCTGTGTCTCCGGGGGAAAGCGCCACCCTCTCTTGTAGGACCAGTCAGAGTGTTAGCAG19830.14044038649081045No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19390.13732421049202295No Hit
GACTTCACTCTCACCATCAGCCGCCTGCAGTCTGAAGATTTTGCGATTTATTACTGTCAGCAGAATGATAGATGG19360.13711174394665107No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18670.13222501340309792No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18390.1302419923129604No Hit
GAATGATAGATGGCCTCCGATATTCACTTTCGGCCCTGGGACCAGAGTCG18040.1277632159502885No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACCCCAGTCGCCCTGGA17540.12422210686075723No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGCGCTCGACTCTGGTCCCAGGGCCGAAAGTGAATATCGGAGG17390.12315977413389784No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17330.12273484104315407No Hit
ACTCTGGTCCCAGGGCCGAAAGTGAATATCGGAGGCCATCTATCATTCTG17240.12209744140703845No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16980.12025606468048218No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16920.11983113158973843No Hit
GCATATTCCAGGGCGACTGGGGTCCCAGCCAGGTTCAGTGGCAGTGGGTC16510.11692742213632278No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACCCCA16400.1161483781366259No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC15350.10871204904861023No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15340.1086412268668196No Hit
GTCAGAGTGTTAGCAGCAACTTAGCCTGGTTCCAGCACCGACCTGGCCAGGCTCCCAGGCTCCTCATCTACGGTG15150.10729560541279771No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15110.10701231668563521No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14690.10403778505042893No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14410.10205476396029142No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14410.10205476396029142No Hit
GTGCTGGAACCAGGCTAAGTTGCTGCTAACACTCTGACTGGTCCTACAAGAGAGGGTGGCGCTTTCCCCCGGAGA14320.1014173643241758No Hit
CTATCATTCTGCTGACAGTAATAAATCGCAAAATCTTCAGACTGCAGGCGGCTGATGGTGAGAGTGAAGTCTGTC14320.1014173643241758No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14250.10092160905164141No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT605.7559755E-662.1431970
GGTATCA21100.040.0240551
GTATCAA51700.037.1599731
GTGGTAT5650.036.9209631
AGGTACG558.789824E-636.700522
GACAGGT3000.034.7672421
GTACGTA702.6722706E-432.4393769
TATCAAC59900.031.9572262
TGGTATC6550.031.8444712
ATCAACG61300.031.3415743
TCAACGC61650.031.279454
AACGCAG62500.031.1771266
CAACGCA63350.030.5994345
GGTACGT803.56467E-529.90828368
ACGCAGA65300.029.7887467
CGCAGAG66050.029.5014448
AGAGTAC67200.028.98425311
GACAGCC2950.027.372791
GCACGAT500.00869521326.94617517
TGGTACG857.667938E-526.7288467
Produced by FastQC (version 0.11.5)