FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704551
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG38250.542898952666308No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33470.47505432537885833No Hit
GATTATTACTGTGCAGCTTGGGATGACAGTATGCCTGGTCTGGTATTCGG32820.46582859154269884No Hit
GCCTTGGGCTGACCGAGGACGGTCAACTTGGTCCCTCCGCCGAATACCAGACCAGGCATACTGTCATCCCAAG27770.3941517363540752No Hit
GTCCTGGGCCCAGTCTGTGCTGAGTCAGCCACCCTCAGCGTCTGGGACCC22180.3148104253631036No Hit
CATCTATACTGATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC21810.3095588537948282No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20030.28429453652042225No Hit
GTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTCTACAGTATTAGTTCCGATGTTGGAG19680.2793268336855671No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19340.2745010652174222No Hit
GAACTAATACTGTAGACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAA18510.2627205127804801No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAACTTGGTCCCTC18330.2601656941796974No Hit
GGCATACTGTCATCCCAAGCTGCACAGTAATAATCAGCCTCATCCTCAGA17790.2525012383773495No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17390.24682386370894369No Hit
GTATTAGTTCCGATGTTGGAGCTGCTTCCGGAACAAGAGATGGAGACCCT17300.2455464544085524No Hit
CAGTATGCCTGGTCTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGT16990.24114648904053787No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGAGTCAGCCACCCTCAGCGTCT15260.21659184359968264No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14230.2019726038285376No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT13600.19303073872579843No Hit
GTGCTGAGTCAGCCACCCTCAGCGTCTGGGACCCCCGGACAGAGGGTCTC13290.1886307733577839No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13240.18792110152423316No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13130.18635982349042157No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12850.18238566122253747No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12830.18210179248911718No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12640.1794050395216244No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12260.17401153358663887No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12220.1734437961197983No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12030.17074704315230552No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11860.16833415891823303No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11660.1654954715840301No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11640.16521160285060982No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11410.16194711241627646No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10910.15485039408076917No Hit
CCCCTGAGGGCCGCTGATCATCAGTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTCTA10830.15371491914708799No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10230.14519885714447925No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10000.1419343667101459No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGAGTCAGCCAC9970.14150856361001546No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG9930.14094082614317488No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT9770.13866987627581254No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT9670.1372505326087111No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC9670.1372505326087111No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA9160.13001187990649365No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9100.12916027370623276No Hit
CTATTTGGACTCTGTGAAGGGCCGATTCACCATTTCCAGAGACAACGCCAAAAATTCAGTGTTTCTACAAATGAG8740.12405063650466751No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCTTGGGATGACAGTATGCCTGGTCTGGTATTCGGCGGAGGGAC8590.12192162100401532No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAACTTGGTC8440.11979260550336313No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAACTTGGTCCCTCCGCCGAATACCAGAC8390.1190829336698124No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC8240.11695391816916022No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8240.11695391816916022No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT8190.1162442463356095No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC8150.1156765088687689No Hit
CCCTCAGCGTCTGGGACCCCCGGACAGAGGGTCTCCATCTCTTGTTCCGG8070.11454103393508774No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC7970.11312169026798627No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC7790.11056687166720366No Hit
CTGTCATCCCAAGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAG7750.10999913420036307No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT7700.10928946236681233No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7660.10872172489997174No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT7610.10801205306642102No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7460.10588303756576883No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC7440.10559916883234854No Hit
AGTCTGTGCTGAGTCAGCCACCCTCAGCGTCTGGGACCCCCGGACAGAGG7440.10559916883234854No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7410.10517336573221811No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA7370.10460562826537752No Hit
ATACCAGACCAGGCATACTGTCATCCCAAGCTGCACAGTAATAATCAGCC7330.10403789079853694No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC7190.10205080966459489No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7190.10205080966459489No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7180.10190887529788475No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAGCTTGGGATGACAGTATGCCTGGT7140.10134113783104416No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7130.10119920346433403No Hit
GACCGAGGACGGTCAACTTGGTCCCTCCGCCGAATACCAGACCAGGCATA7120.10105726909762389No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7120.10105726909762389No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGTAGAAACA7110.10091533473091373No Hit
CCCTAAGACTCTCCTGTGAAGCCTCTGGATTCTCTCTTGACAATTATTGG7110.10091533473091373No Hit
ATACTGATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG7050.10006372853065286No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTGC200.00725056651.942543
CGAACTG351.7368844E-551.57435262
TCAAACG351.8889818E-550.847957
AGGGGGG3550.049.16711870
CGCGTAT352.3176386E-549.12002639
GGTATCA6200.047.050361
GCGTATG306.3699007E-445.9114640
CCGGCTT404.696024E-543.5856447
ATACGTA405.5492474E-542.3699411
TACGTAC405.5492474E-542.3699412
CTCTTAT1400.041.1886021
ATGCCGG602.7817077E-740.47896644
GCTTAAA350.001257428139.9869449
ATCGCAC350.001469672838.7382319
TTATACA4250.038.2797742
ACAGATC656.220689E-736.5292631
TCTCGTA2850.036.13127538
ACGAACT400.002105299236.0040761
CGTATGC3550.035.9845341
TATGCCG4000.035.4940443
Produced by FastQC (version 0.11.5)