FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704551
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42490.60307912415141No Hit
GATTATTACTGTGCAGCTTGGGATGACAGTATGCCTGGTCTGGTATTCGG31240.44340296160249576No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30680.43545463706672766No Hit
GTCCTGGGCCCAGTCTGTGCTGAGTCAGCCACCCTCAGCGTCTGGGACCC24580.3488746733735386No Hit
GCCTTGGGCTGACCGAGGACGGTCAACTTGGTCCCTCCGCCGAATACCAGACCAGGCATACTGTCATCCCAAG24100.3420618237714516No Hit
CATCTATACTGATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC23430.33255222120187183No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20850.29593315459065417No Hit
GAACTAATACTGTAGACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAA19970.2834429303201613No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG19660.27904296495214687No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19510.2769139494514946No Hit
GTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTCTACAGTATTAGTTCCGATGTTGGAG19250.27322365591703085No Hit
GTATTAGTTCCGATGTTGGAGCTGCTTCCGGAACAAGAGATGGAGACCCT16960.2407206859404074No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGAGTCAGCCACCCTCAGCGTCT16850.23915940790659582No Hit
CAGTATGCCTGGTCTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGT16380.232488492671219No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAACTTGGTCCCTC16060.2279465929364943No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15420.21886279346704499No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14930.2119080094982478No Hit
GTGCTGAGTCAGCCACCCTCAGCGTCTGGGACCCCCGGACAGAGGGTCTC14630.20764997849694344No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13450.19090172322514623No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13110.18607595475700126No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12810.1818179237556969No Hit
GGCATACTGTCATCCCAAGCTGCACAGTAATAATCAGCCTCATCCTCAGA12230.17358573048650844No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12180.17287605865295771No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12040.17088897751901566No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11560.16407612791692866No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11500.16322452171666776No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG11300.16038583438246484No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT11260.15981809691562426No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10960.15556006591431992No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGAGTCAGCCAC10870.1542826566139286No Hit
CCCCTGAGGGCCGCTGATCATCAGTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTCTA10600.15045042871275463No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10470.14860528194552275No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10330.14661820081158072No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10300.1461923977114503No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA10180.14448918531092853No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG10070.1429279072771169No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCTTGGGATGACAGTATGCCTGGTCTGGTATTCGGCGGAGGGAC9830.13952148247607343No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9750.13838600754239225No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9370.1329925016074067No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT9320.13228282977385597No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9060.12859253623939218No Hit
CCCTCAGCGTCTGGGACCCCCGGACAGAGGGTCTCCATCTCTTGTTCCGG8940.12688932383887042No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT8880.12603771763860958No Hit
CTATTTGGACTCTGTGAAGGGCCGATTCACCATTTCCAGAGACAACGCCAAAAATTCAGTGTTTCTACAAATGAG8810.12504417707163853No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8800.12490224270492838No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC8780.1246183739715081No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8600.12206355537072545No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC8510.12078614607033415No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8500.12064421170362401No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAACTTGGTCCCTCCGCCGAATACCAGAC8430.11965067113665298No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT8240.11695391816916022No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8230.11681198380245007No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA8010.11368942773482685No Hit
AGTCTGTGCTGAGTCAGCCACCCTCAGCGTCTGGGACCCCCGGACAGAGG7950.112837821534566No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC7820.11099267476733408No Hit
ATACTGATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG7790.11056687166720366No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC7770.11028300293378336No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC7640.10843785616655147No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAACTTGGTC7590.10772818433300074No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7570.10744431559958044No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7510.10659270939931957No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG7460.10588303756576883No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT7390.10488949699879782No Hit
ACTGTAGACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCAT7310.10375402206511664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)