FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1343210
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT80420.5987150185004578No Hit
CTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA79290.590302335450153No Hit
ATATTATACTACTCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAACCGAACTGTGGCTGCACCAT77300.5754870794589082No Hit
GTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC57160.4255477550048019No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56010.41698617490935896No Hit
GTTTAGGAGGCTGTCGTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAAGACT53030.39480051518377623No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50160.3734337892064532No Hit
GTATAAAAGACTCTGGCTGGACTGGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA40220.299431957772798No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38160.2840955621235697No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37400.27843747440832034No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35920.2674190930680981No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32440.24151100721406185No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30630.22803582462906022No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30000.22334556770720887No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29460.2193253474884791No Hit
ATACTACTCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAACCGA29020.21604961249544002No Hit
GAGTAGTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT27890.20763692944513515No Hit
GTTTATTACTGTCAGCAATATTATACTACTCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAACCGAA27800.20696689274201352No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26570.19780972446601797No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26430.196767445150051No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25690.19125825447993985No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24670.18366450517789476No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24410.18172884359109895No Hit
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA23990.178602005643198No Hit
GTGCAGCCACAGTTCGGTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC23930.1781553145077836No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23850.17755972632723102No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23600.17569851326300429No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC22670.16877480066408082No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCCAGTCCAGCCAGAGTCTTTTATA22660.16870035214151174No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCCAGTCCAGCCAGAGTCTTTTATACAGCTCCAA21340.15887314716239456No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA20480.15247057422145457No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20350.15150274342805667No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20330.15135384638291854No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCCAGTCCAGC20080.14949263331869178No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19860.14785476582217225No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19790.14733362616418877No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19620.1460680012805146No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACT19530.14539796457739296No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19080.14204778106178484No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC18770.13973987686214367No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18770.13973987686214367No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18680.13906984015902205No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18650.13884649459131485No Hit
GTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC17370.1293170837024739No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16850.1254457605288823No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCGTGGTTTCTGCTGGTA16720.1244779297354844No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16310.12142554031015255No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCCAGTCCAGCCAGAGTCTTTTATACAGCTCCAACAATAAGAACTA16130.1200854669039093No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT16060.1195643272459258No Hit
GGCAGGGGCAGCAAGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTT15610.11621414373031767No Hit
CTACTTAGCTTGGTACCAGCAGAAACCACGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAATC15460.11509741589178163No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACTCCTTGGACGTT15220.11331065135012396No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15150.11278951169214047No Hit
CAGCAAGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA14860.11063050453763744No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT14630.10891818851854886No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14500.10795035772515095No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14500.10795035772515095No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14450.1075781151123056No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14430.10742921806716746No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14420.10735476954459838No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14400.10720587249946024No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14350.1068336298866149No Hit
ACTTAGCTTGGTACCAGCAGAAACCACGACAGCCTCCTAAACTGCTCATT14320.10661028431890769No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14250.1060891446609242No Hit
AGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTCCGGGGACGTCGTGATGA14210.10579135057064792No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14090.1048979682998191No Hit
GTGTATTACTGTGCGAAAGATCGCTACGATATTTTGACTGGCCAAAACAG13980.10407903455155934No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13850.10311120375816143No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13760.1024411670550398No Hit
GGATCTCTGGTGCCTCCGGGGACGTCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG13680.10184557887448722No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACTCCTT13630.1014733362616419No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA352.5882307E-548.2151874
GGTATCA10100.040.7954251
CGTATGC3900.039.7857341
GTGGTAT2950.038.9251171
TCGTATG3750.038.54977440
ATCTCGT3350.036.7605937
GATCTCG3650.036.49569336
CGAGACA5100.035.76791823
GACAGGT3250.035.3320271
GTATGCC4650.034.17388542
ATGCCGT3900.033.8089944
GGGCGTA701.1561824E-633.7506333
TAGACTC400.002891637233.750634
ATCTCCG5250.033.429210
TGGTATC3450.033.2614972
GTATCAA21700.033.1507151
CACGAGA5500.033.1505621
GGTCTGG34700.033.09280870
ACGAGAC5550.032.85802522
CTTATAC5600.032.5670741
Produced by FastQC (version 0.11.5)