FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1343210
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTACTCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAACCGAACTGTGGCTGCACCAT78250.5825596891029697No Hit
CTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT74390.5538225593913089No Hit
CTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA67750.5043887404054467No Hit
GTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC66730.4967949911034016No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58990.43917183463494164No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50460.37566724488352526No Hit
GTTTAGGAGGCTGTCGTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAAGACT49380.36762680444606577No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42490.3163317723959768No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38500.28662681189091804No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37310.27776743770519874No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35440.2638455639847827No Hit
GAGTAGTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT32260.2401709338078186No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32160.23942644858212786No Hit
GTATAAAAGACTCTGGCTGGACTGGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA31450.23414060347972396No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28130.20942369398679284No Hit
GTTTATTACTGTCAGCAATATTATACTACTCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAACCGAA28100.2092003484190856No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27970.2082325176256877No Hit
ATACTACTCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAACCGA27430.20421229740695798No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA26630.1982564156014324No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25870.1925983278861831No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25790.19200273970563056No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25060.18656799755808845No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24930.18560016676469054No Hit
GTGCAGCCACAGTTCGGTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC24920.18552571824212147No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24460.18210108620394427No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23590.1756240647404352No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22900.17048711668316943No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22590.16817921248352827No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22440.1670624846449922No Hit
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA21650.16118105136203573No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21510.16013877204606874No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCCAGTCCAGCCAGAGTCTTTTATA21010.15641634591761527No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC20620.15351285353742153No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCCAGTCCAGCCAGAGTCTTTTATACAGCTCCAA20590.15328950796971436No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19780.1472591776416197No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19490.14510017048711668No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACT19130.1424200236746302No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG19070.14197333253921576No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT19040.14174998697150856No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18530.137953112320486No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17880.1331139583534965No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC17780.13236947312780578No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17600.13102939972156252No Hit
GTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC17560.13073160563128625No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17250.12842370143164508No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17180.1279025617736616No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16740.12462682678062253No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16570.12336120189694835No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCCAGTCCAGC16260.12105329769730719No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC16120.12001101838134023No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA16050.11948987872335674No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16040.11941543020078767No Hit
GTGTATTACTGTGCGAAAGATCGCTACGATATTTTGACTGGCCAAAACAG15850.11800090827197535No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15840.11792645974940627No Hit
GGCAGGGGCAGCAAGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTT15790.11755421713656093No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCGTGGTTTCTGCTGGTA15780.11747976861399186No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGGTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCAAGGAGTAGT15600.1161396952077486No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15130.11264061464700233No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14510.10802480624772001No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT14420.10735476954459838No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACTCCTT14410.10728032102202932No Hit
CAGCAAGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA14380.1070569754543221No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14300.10646138727376955No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14240.10601469613835514No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACTCCTTGGACGTT14140.10527021091266443No Hit
AGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTCCGGGGACGTCGTGATGA14010.10430238011926653No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13960.10393013750642117No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13860.10318565228073048No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13690.10192002739705631No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13670.10177113035191818No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13510.10057995399081306No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCCAGTCCAGCCAGAGTCTTTTATACAGCTCCAACAATAAGAACTA13460.10020771137796769No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)