FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1255806
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG94200.7501158618449028No Hit
GATTATTACTGTGCAACGTGGGATGACAGCCTGACTGGTCCGGTGTTCGG59800.47618820104379184No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGTCCACTGATGGCCAGGGAGG54020.43016198361848884No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36500.2906499889314114No Hit
GTCCTGGGCCCAGTCTGTTCTGACTCAGCCACCCTCAGCGTCTGGGACCC32760.26086831883268596No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31590.25155159316009No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29360.23379407328839008No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGG28200.22455697774974798No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25350.20186238957291175No Hit
GTATTATACTGGGAACACTTATTACAACCCGTCCCTCGCGAGTCGAGTCTCCATATCCGGAGACACTTCCAAGAA24180.19254566390031583No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23830.18975860921193244No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTTCTGACTCAGCCACCCTCAGCGTCT23550.1875289654612257No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC21750.17319554134953966No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20740.1651528978202047No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20510.16332140473926704No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20120.1602158295150684No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG20110.1601361993811146No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19410.1545620900043478No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18210.14500647392989044No Hit
GTTCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC18120.14428980272430614No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCAGTCAGG18100.14413054245639853No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17650.14054718642847702No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17440.13887495361544697No Hit
GTAATAATGAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG16940.1348934469177564No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGACTCAGCCAC16770.1335397346405416No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16280.12963785807680486No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15820.12597487191492954No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15730.12525820070934524No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG15530.12366559803026901No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15290.12175447481537753No Hit
GTATAATACATATTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAATAATAACTACTT15130.12048039267211655No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15090.12016187213630132No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15070.12000261186839369No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14570.11602110517070312No Hit
CATTATTACTATAGATGAGGAGTTTGGGGGCCGTGCCTGGGAGTTGCTGG14560.11594147503674931No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG14440.11498591342930357No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14160.11275626967859685No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGCGAAAATACTGTAAATTGGTACCAGCAACTCCCAGGCACGGCCCC13750.1094914341864906No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT13480.10734142056973768No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC13290.10582844802461526No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13120.10447473574740047No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13100.10431547547949285No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12840.10224509199669375No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA12700.10113027012134039No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12690.10105063998738657No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12630.10057285918366372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGAG21650.042.06561370
GGTATCA16450.040.8865361
GTATCAA42950.039.93221
GTGGTAT5050.036.625841
GTCCTAG27650.035.11513570
TGGTATC5250.034.5941472
TATCAAC50500.033.8332
ATCAACG52300.032.668573
TCAACGC52650.032.4514054
CAACGCA53300.031.9307215
AACGCAG53200.031.927526
GACAGGT2300.030.70491
CGCAGAG55300.030.4717968
ACGCAGA56050.030.3040877
AGAGTAC56250.030.00858511
CAGAGTA56750.029.80938110
GCAGAGT57450.029.5047119
GTGGCCG8000.028.2248170
AATCAGC21500.027.2205096
AATAATC21150.027.1927993
Produced by FastQC (version 0.11.5)