EGAF00004689377 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00004689377
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1194497
Sequences flagged as poor quality	0
Sequence length	20-76
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCATAATAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT	4699	0.3933873421197374	No Hit
CTATTATGCTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC	4640	0.38844802456598887	No Hit
GTAATATACGGCGGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATACA	4489	0.37580672031825946	No Hit
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG	3989	0.33394809698140726	No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT	3245	0.27166246545617107	No Hit
GTATATTACTGTGCTAAAGGCGAAAAGAGAGCCTCTGGTGGGGTAGTAGT	2959	0.24771933290749162	No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC	2897	0.24252886361372192	No Hit
GATCTATGCTGCATCCAGTTTGCACAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA	2677	0.22411106934550695	No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG	2344	0.19623322620316336	No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC	2202	0.1843453771754973	No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG	2112	0.1768108249748639	No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA	1983	0.16601130015395602	No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG	1909	0.1598162239001019	No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCAGAAG	1813	0.15177936821942625	No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA	1753	0.146756333419004	No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC	1746	0.14617031269228806	No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT	1728	0.14466340225216137	No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG	1727	0.14457968500548765	No Hit
GCATAGATCAGGCGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAGCC	1724	0.14432853326546655	No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG	1711	0.1432402090587084	No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC	1634	0.13679398106483315	No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT	1629	0.13637539483146463	No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC	1624	0.1359568085980961	No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT	1608	0.13461733265131684	No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC	1572	0.13160351177106347	No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT	1550	0.12976173234424196	No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTGACCACTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGG	1545	0.12934314611087347	No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG	1542	0.12909199437085234	No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT	1488	0.12457126305047228	No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG	1482	0.12406895957043007	No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT	1471	0.12314806985701932	No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT	1463	0.12247833188362967	No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG	1444	0.12088770419682929	No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT	1416	0.11854362128996555	No Hit
ACTTATTACTGTCTACAGCATAATAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA	1385	0.11594838664308073	No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT	1298	0.10866498618246843	No Hit
GTCCATGTAGTGGTCACTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTC	1297	0.10858126893579473	No Hit
GTGCAAACTGGATGCAGCATAGATCAGGCGCTTAGGGGCTTTCCCTGGCT	1283	0.10740922748236287	No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAGGGGA	1270	0.10632090327560471	No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG	1265	0.10590231704223618	No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGTGACAT	1262	0.10565116530221506	No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT	1254	0.10498142732882546	No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT	1240	0.10380938587539357	No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC	1213	0.10154902021520355	No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTACAGCATAATAGTTAC	1208	0.10113043398183504	No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG	1208	0.10113043398183504	No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA	1205	0.10087928224181392	No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC	1198	0.10029326151509799	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATTCG	20	0.0067492407	52.8945	53
GTCGTAT	145	0.0	52.18836	39
TGCTCGA	20	0.007235386	51.97232	44
TATGGCG	35	2.2351242E-5	49.42564	43
TCGTATG	390	0.0	45.907288	40
ATGTCGT	150	0.0	45.711243	37
GATGTCG	165	0.0	45.668762	36
CGTATGC	410	0.0	45.42267	41
ATGCCGT	415	0.0	45.084423	44
TGTCGTA	160	0.0	45.077053	38
GCGATGT	180	0.0	43.669975	34
TGAGCGA	540	0.0	43.559814	31
TATACAC	570	0.0	43.47124	3
TCTCCGA	560	0.0	43.03345	11
GAGCGAT	500	0.0	42.995342	32
CACGAGA	555	0.0	42.82212	21
ATCTCCG	565	0.0	42.652622	10
CCCACGA	585	0.0	42.361916	19
CTTATAC	570	0.0	42.296185	1
ACGAGAC	565	0.0	42.069496	22

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content