FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194497
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48710.40778670854761456No Hit
CTATTATGCTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC47360.3964848802466645No Hit
GCATAATAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT43070.3605701814236453No Hit
GTAATATACGGCGGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATACA42980.35981672620358196No Hit
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG35800.2997077430918621No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33720.2822945557837316No Hit
GTATATTACTGTGCTAAAGGCGAAAAGAGAGCCTCTGGTGGGGTAGTAGT28570.23918017374677372No Hit
GATCTATGCTGCATCCAGTTTGCACAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA24630.2061955785573342No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23850.19966563331678522No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21200.17748056294825354No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21120.1768108249748639No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20620.1726249626411787No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT19350.1619928723136182No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17620.14750978863906733No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16670.13955665020506539No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16300.13645911207813832No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT16130.13503591888468536No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCAGAAG16100.13478476714466422No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16060.13444989815796943No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTGACCACTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGG15920.13327785670453757No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15830.13252440148447422No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15760.1319383807577583No Hit
GCATAGATCAGGCGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAGCC15370.12867340813748382No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC15310.12817110465744158No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15290.12800367016409417No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT14990.12549215276388304No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14770.12365037333706154No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC14460.1210551386901767No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14070.11779016606990222No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14070.11779016606990222No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13940.11670184186314406No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13530.1132694347495222No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13500.11301828300950108No Hit
GTGCAAACTGGATGCAGCATAGATCAGGCGCTTAGGGGCTTTCCCTGGCT13360.11184624155606922No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG12960.10849755168912104No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12920.10816268270242621No Hit
GTCCATGTAGTGGTCACTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTC12810.10724179298901547No Hit
ACTTATTACTGTCTACAGCATAATAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA12630.10573488254888878No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12610.10556744805554137No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12550.10506514457549913No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12380.10364195138204617No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGA200.006754780752.88352253
TCTTGAG14450.042.75010370
TAACAGT15700.040.2614770
GTGGTAT3800.036.5288661
CTCTTGA14150.036.05180469
GTATCAA26950.035.3007131
TGTTAGA10700.031.55154870
TATCAAC30000.031.370622
AATCATA450.004383359631.00174548
ATCAACG31050.030.309783
AACGCAG31500.029.9867656
TCAACGC31600.029.7847294
CAACGCA31650.029.7376795
GACAGGT2900.029.186231
CGCAGAG33100.028.6395388
ACGCAGA33400.028.3822967
ATCTCTT15950.027.90423867
TACTGGG16050.027.74746170
GACAGAC1353.8198777E-1027.5863881
CCCATGC2700.027.584083
Produced by FastQC (version 0.11.5)