FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504266
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC46360.30819017381234437No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA45720.30393560713331286No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT39590.26318483566071427No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG38730.2574677616857657No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34320.22815113816306423No Hit
GTTCTGGGGCTGAGCGCTCCCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGGTGACTATTACTGTCAGAC34310.22808466055870436No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT31320.20820785685510407No Hit
CTATTACTGTCAGACCTGGGGCACTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC31250.207742513624585No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31140.20701125997662648No Hit
GCCCCAGAACTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCT31030.20628000632866794No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATGCCAGTGCCCCAGGTCTGACAGTAATAGTCAC30500.20275669329759496No Hit
GAAATATACGGCCGTGTCCGCGGCTCGCAGGCTGTTCATTAGCAGATACA30350.20175952923219698No Hit
GGGTCTCTCTCCCAGCTTCTCCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC29930.19896746984908253No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC29720.19757144015752534No Hit
CAGTAATAGTCACCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGGAGCGCTCAGCCCCAGAACTGGAGC28680.19065776930409914No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA27050.17982191979344078No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT25860.1719110848746166No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24720.16433263797759173No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGA24550.163202518703474No Hit
CAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGTTCTGGGGCTGAGCGCTCCCTCACCATCTCCAG24320.16167353380319704No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG24060.1599451160898405No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG24060.1599451160898405No Hit
CCTGCACTCTGAGCAGTGGGCACAGCACCTACGCCATCGCATGGCATCAG23350.15522520618028993No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22910.15230019158845576No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22470.1493751769966216No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22290.148178580118144No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCACCTACGCCATCGCATGGCATC22250.14791266970070452No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21930.1457853863611888No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21760.14465526708707105No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21400.1422620733301158No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21360.14199616291267633No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21350.14192968530831648No Hit
GCACAGGGTCTCTCTCCCAGCTTCTCCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG21280.1414643420777974No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21110.14033422280367966No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC20590.13687738737696659No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG20260.13468362643309095No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20240.13455067122437123No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT20180.13415180559821202No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19830.13182508944561666No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19610.1303625821496996No Hit
GTTCTTGCTGAGGCTGAACAATGATGGCAGTCACAGCAAGGGGGACGGGA19390.1289000748537825No Hit
CCCAAATGCCAGTGCCCCAGGTCTGACAGTAATAGTCACCCTCATCCTCA19380.12883359724942264No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT19380.12883359724942264No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19140.12723813474478582No Hit
GTATATTTCTGTGCGAAAGCCCCCGCTCGAGGTGGCGTCGCTGACTACTGGGGCCAGGGAACCCTGGTCACCGT18990.1262409706793878No Hit
CTCCCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGGTGACTATTACT18840.12524380661398982No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18730.12451255296603127No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18670.12411368733987206No Hit
GTGTATCTGCTAATGAACAGCCTGCGAGCCGCGGACACGGCCGTATATTT18660.12404720973551221No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA18310.12172049358291684No Hit
GTAATAGTCACCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGGAGC18130.12052389670443925No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17630.11720001648644587No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC17580.11686762846464654No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTCTCCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC17440.11593694200360839No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16950.11267953938997491No Hit
GGACTGGAGTGGGTCTCAGCTCTTAGTGGTAGTGGTGGTAGCACATACTA16710.1110840768853381No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16700.11101759928097822No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16690.11095112167661836No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16690.11095112167661836No Hit
GCCCTGGGACTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGA16590.11028634563301969No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16390.10895679354582236No Hit
GGGTTCCCTGGCCCCAGTAGTCAGCGACGCCACCTCGAGCGGGGGCTTTCGCACAGAAATATACGGCCGTGTCCG16250.1080261070847842No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTCTCCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT16080.10689598781066646No Hit
CTCCAGTCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCATAGCTGC16000.10636416697578752No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC15940.10596530134962834No Hit
CCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGGAGCGCTCAGCCCC15910.10576586853654872No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15790.10496813728423032No Hit
TCCTCAGACTGGAGGCTGGAGATGGTGAGGGAGCGCTCAGCCCCAGAACTGGAGCCTGAGAAGCGATCAGGGATC15770.1048351820755106No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT15650.10403745082319217No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC15530.10323971957087376No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15500.10304028675779417No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGT15420.10250846592291522No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15240.10131186904443763No Hit
GTCTGAGGATGAGGGTGACTATTACTGTCAGACCTGGGGCACTGGCATTTGGGTGTTCGGCGGAGGGACCAAG15170.10084652581391854No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT15150.10071357060519881No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15070.10018174977031989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG5900.072.2580270
GTCTATA200.00670593152.98137756
CGTATTT200.007783924751.0211910
CGGGATA352.4711051E-548.5948417
GGTATCA10400.045.1476361
ATCTCGT6350.043.25794637
GCCGTCT7000.043.17895546
TCTCGTA6650.042.3903338
CGTATGC7400.041.98116341
CATCTCG6500.041.6796936
TCGTATG7200.041.6430440
GACAGGT3050.041.2753871
CTTATAC8100.040.745161
ATGCCGT7450.040.46778544
CGAGACG7750.040.38611623
CACGAGA7850.040.30235321
CGTCTTC7350.040.2855248
CTACATC6900.040.1525333
CCGTCTT7450.040.15221447
TTATACA8350.039.9194452
Produced by FastQC (version 0.11.5)