FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889209
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33110.3723534062295816No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29590.3327676620457058No Hit
CAATAAGACCTACTTAGCTTGGTACCAACAAAGACCAGGACAGCCTCCTAAGGTACTCATTTACTGGGCTTCTAC27060.30431540841354504No Hit
GTTTATTACTGTCAGCAATATTATCTTTCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA26360.29644324337697886No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21100.23728954610220993No Hit
CCTTAGGAGGCTGTCCTGGTCTTTGTTGGTACCAAGCTAAGTAGGTCTTATTGTTGCCACTAAATAAAAGACT20860.23459051808967296No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20800.2339157610865387No Hit
GTAATAAACAGCCGCATCTTCAGCCTGCAGCCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGGCA19850.22323210853691314No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19090.2146851864972127No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18850.21198615848467572No Hit
CTCCTAAGGTACTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCT18030.20276447944184103No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17580.19770380191833414No Hit
ATATTATCTTTCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT17280.19433001690266294No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16840.18938179887967846No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16560.186232932865052No Hit
GTCTCCAGGCTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT16440.18488341885878348No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16010.18004766033632139No Hit
GACCAGGACAGCCTCCTAAGGTACTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTG15640.17588665881699353No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15300.1720630357992328No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14800.1664400607731141No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC14780.16621514177206934No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14540.16351611375953234No Hit
GTAGTACACGGCTGTGTCCTCTGTCTTCAGGTTGTTCATTTGCAGATATA14350.16137938324960724No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14090.1584554362360255No Hit
GTCCCTGACCGATTCAGTGCCAGCGGGTCTGGGACAGATTTCACTCTCAC13610.15305738021095153No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13470.1514829472036383No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC13440.15114556870207116No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG13260.14912129769266844No Hit
CCCTTAGACTCTCTTGTGCAGGCTCAGGTTTCACTTTCAGAAACGCCTGG13130.14765932418587757No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13000.1461973506790867No Hit
CTCCAGGCTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC12620.14192388965923647No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT12570.14136159215662458No Hit
GATTTCACTCTCACCATCAGCAGGCTGCAGGCTGAAGATGCGGCTGTTTATTACTGTCAGCAATATTATCTTTCT12030.13528877912841636No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11990.13483894112632688No Hit
GAGTGAAATCTGTCCCAGACCCGCTGGCACTGAATCGGTCAGGGACCCCG11980.1347264816258045No Hit
GTCTTATTGTTGCCACTAAATAAAAGACTCTGGCTGGACTGGCAGTTGAT11930.13416418412319264No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11780.132477291615357No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11610.13056548010647664No Hit
ATTCAGTGCCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGGC11410.12831629009602916No Hit
GTGTACTACTGTGCCACAGTCAAGGCATATTATGAGAATAGTGGCTATTTCTCAAATTGGTTCGACCCCTGGGGC11100.12483004557983556No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11060.12438020757774607No Hit
GTCCTGGTCTTTGTTGGTACCAAGCTAAGTAGGTCTTATTGTTGCCACTA11040.1241552885767013No Hit
AGATAATATTGCTGACAGTAATAAACAGCCGCATCTTCAGCCTGCAGCCTGCTGATGGTGAGAGTGAAATCTGTC10990.12359299107408944No Hit
GAATCGGTCAGGGACCCCGGATTCCCGGGTAGAAGCCCAGTAAATGAGTA10880.12235593656834333No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10850.12201855806677621No Hit
GACCGATTCAGTGCCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGGCTGCAGGCTGAAGATGCGG10640.11965690855580634No Hit
GTCCCAGACCCGCTGGCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT10590.11909461105319447No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10530.11841985405006022No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10510.11819493504901547No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT10500.11808247554849309No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10430.11729525904483648No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10350.11639558304065749No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACGCAGTCTCCAGGCTCCCTGGCTGTGTCTCTGGGCGAGAGGG10320.11605820453909035No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10310.115945745038568No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10160.11425885253073237No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCCAGTCCAGC10120.11380901452864288No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGGTGGATTTTC10090.11347163602707575No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10070.11324671702603101No Hit
CTTTTATTTAGTGGCAACAATAAGACCTACTTAGCTTGGTACCAACAAAG10050.11302179802498626No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9960.11200966252028488No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9760.10976047250983739No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCCAGTCCAGCCAGAGTCTTTTATTTAGTGGCAACAATAAGACCTA9640.1084109585035689No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9630.10829849900304653No Hit
CATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGCCAGCGGGTCTGGGACAGATTTCA9500.10683652549625566No Hit
GTGCCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGGCTGCAGGCTGAAGATGCGGCTGTTTATTA9400.10571193049103192No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9270.10424995698424105No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9200.10346274048058442No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9100.10233814547536069No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9070.10200076697379355No Hit
ACTTAGCTTGGTACCAACAAAGACCAGGACAGCCTCCTAAGGTACTCATT9070.10200076697379355No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9020.10143846947118168No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9000.10121355047013694No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8950.10065125296752507No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)