FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773499
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT42230.5459606282619629No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31300.40465469250768266No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25800.3335492353577703No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGTTA24330.3145446859013392No Hit
GTAATACACAGCCGTGTCCTCAGCTGTCAGGCTGTTCATTTGCAGAAACA22310.2884295907299169No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21900.2831290021060143No Hit
GTCTAGAGCCTGAAGATTCTGCAGTTTATTACTGTCAGCAACGTAGTGAT20500.2650294311951276No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19850.2566260589865016No Hit
GTTTATTACTGTCAGCAACGTAGTGATTGGCCTCCTGTCACCTTCGGCCA18280.23632868303643573No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGTCTAGAGCCTGAAGATT15990.20672295633219953No Hit
GTAATAAACTGCAGAATCTTCAGGCTCTAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA15900.20555941248792822No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15800.20426658599429345No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15330.19819030147421005No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTCGTGTCC15030.19431182199330574No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14830.1917261690060362No Hit
GTCCTTGTCTCCAGGGCAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCTACTTAGCCTGGTA14820.19159688635667274No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACGCCAGGGGCCCTGTG14800.1913383210579458No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14680.18978692926558405No Hit
GTCTCCAGCCACCCTGTCCTTGTCTCCAGGGCAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAC14270.1844863406416815No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14220.18383992739486413No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14130.18267638355059282No Hit
GTACCAACAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCCATGATGCATCCCACAGGGCCCCTGGCGTCCCAGC14060.1817714050050485No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13770.17802220817350764No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACCTACTTAGCCTGGTACCAACAGAGACCTGGCCAGGCTCCCAGGCT13750.1777636428747807No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13380.17298018484833205No Hit
GTGTATTACTGTGCGAGAGATCCCCTAGTAACGTTAGAGGCGGTGCGTGCTTCCTTTTACAACGGTATGGACGT13350.17259233690024164No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13040.16858457476997385No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12840.1659989217827043No Hit
GACTTCACTCTCACCATCAGCAGTCTAGAGCCTGAAGATTCTGCAGTTTATTACTGTCAGCAACGTAGTGATTGG12770.16509394323715995No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12590.1627668555486174No Hit
GTGTTAGCACCTACTTAGCCTGGTACCAACAGAGACCTGGCCAGGCTCCC12280.1587590934183496No Hit
GTCAGAGTGTTAGCACCTACTTAGCCTGGTACCAACAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCCATGATG12030.15552702718426267No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT11760.1520363956514488No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACGCCA11200.1447965672870941No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11000.14221091429982455No Hit
GCTCTAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACGCCAGGGG10940.1414352184036437No Hit
GTCGTGTCCCTTGGCCGAAGGTGACAGGAGGCCAATCACTACGTTGCTGA10820.139883826611282No Hit
ACAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCCATGATGCATCCCACAGGGCCCCTGGCGTCCCAGCCAGGTT10510.1358760644810142No Hit
CTGTTGGTACCAGGCTAAGTAGGTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTCTGCCCTGGAGA10080.13031691055838468No Hit
CCTGTCACCTTCGGCCAAGGGACACGACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG9860.1274726922723882No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9770.1263091484281169No Hit
GGACACGACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC9730.125792017830663No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC9710.12553345253193604No Hit
CAGTAATAAACTGCAGAATCTTCAGGCTCTAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9500.12281851689530303No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9370.12113784245357784No Hit
GGTGACAGGAGGCCAATCACTACGTTGCTGACAGTAATAAACTGCAGAAT9320.12049142920676045No Hit
TCCATGATGCATCCCACAGGGCCCCTGGCGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT9280.11997429860930654No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCCTTGTCTCCAGGGCAGAGAGC9120.1179057762194909No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9090.11751792827140047No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8990.11622510177776571No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8870.11467370998540398No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8840.11428586203731356No Hit
CAGTAATACACAGCCGTGTCCTCAGCTGTCAGGCTGTTCATTTGCAGAAA8800.11376873143985965No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCTACTTAGCCTGGTACCAACAGAGACCTGGCCAGG8780.11351016614113268No Hit
TACTTAGCCTGGTACCAACAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCCATGATGCATCCCACAGGGCCC8770.11338088349176921No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8730.11286375289431533No Hit
CTCCAGGCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGCATAGGTAC8700.11247590494622489No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8620.11144164375131707No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGTC8380.10833886016659362No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8110.10484822863377975No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8060.10420181538696237No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8050.1040725327375989No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCC8020.10368468478950846No Hit
CTGCTACTCTGGCTCCCAGATGTTACCGGAGAAGTTGTGTTGACACAGTC7940.10265042359460064No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7800.10084046650351197No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTCGTGTCCCTTGGCCGAAGGTGACAGGAGGCCAATCACTACGTTG7750.10019405325669459No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)