FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773499
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86651.120234156734527No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31870.4120238035214008No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGTTA23910.3091148146280732No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21440.27718200023529443No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20820.26916647597475885No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20190.2610216690648598No Hit
GTCTAGAGCCTGAAGATTCTGCAGTTTATTACTGTCAGCAACGTAGTGAT19500.2521011662587799No Hit
GTAATACACAGCCGTGTCCTCAGCTGTCAGGCTGTTCATTTGCAGAAACA19450.2514547530119625No Hit
GTTTATTACTGTCAGCAACGTAGTGATTGGCCTCCTGTCACCTTCGGCCA18340.23710437893261657No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16890.21835839477491245No Hit
GTAATAAACTGCAGAATCTTCAGGCTCTAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16730.2162898723850968No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16690.2157727417876429No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGTCTAGAGCCTGAAGATT16350.21137713170928468No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15350.198448866772937No Hit
GTCCTTGTCTCCAGGGCAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCTACTTAGCCTGGTA15310.1979317361754831No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14840.1918554516553997No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTCGTGTCC14480.1872012762783145No Hit
GTCTCCAGCCACCCTGTCCTTGTCTCCAGGGCAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAC14370.18577916713531625No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACGCCAGGGGCCCTGTG13980.1807371438101407No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13730.17750507757605374No Hit
GTGTATTACTGTGCGAGAGATCCCCTAGTAACGTTAGAGGCGGTGCGTGCTTCCTTTTACAACGGTATGGACGT13600.17582440313432857No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13370.1728509021989686No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13310.17207520630278772No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACCTACTTAGCCTGGTACCAACAGAGACCTGGCCAGGCTCCCAGGCT13150.17000668391297208No Hit
GTACCAACAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCCATGATGCATCCCACAGGGCCCCTGGCGTCCCAGC12970.16767959622442952No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12760.1649646605877965No Hit
GACTTCACTCTCACCATCAGCAGTCTAGAGCCTGAAGATTCTGCAGTTTATTACTGTCAGCAACGTAGTGATTGG12620.16315470349670783No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12550.16224972495116347No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12440.16082761580816524No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12260.15850052811962265No Hit
GTCAGAGTGTTAGCACCTACTTAGCCTGGTACCAACAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCCATGATG12210.15785411487280526No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT12190.15759554957407831No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACGCCA11050.14285732754664193No Hit
GCTCTAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACGCCAGGGG10980.1419523490010976No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCCTTGTCTCCAGGGCAGAGAGC10370.13406610738992553No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10360.13393682474056204No Hit
GGACACGACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC10250.1325147155975638No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10110.13070475850647512No Hit
GTCGTGTCCCTTGGCCGAAGGTGACAGGAGGCCAATCACTACGTTGCTGA10050.12992906261029424No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9810.1268262790255708No Hit
GTGTTAGCACCTACTTAGCCTGGTACCAACAGAGACCTGGCCAGGCTCCC9750.12605058312938994No Hit
ACAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCCATGATGCATCCCACAGGGCCCCTGGCGTCCCAGCCAGGTT9730.125792017830663No Hit
CAGTAATAAACTGCAGAATCTTCAGGCTCTAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9520.12307708219402999No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9400.12152569040166826No Hit
CCTGTCACCTTCGGCCAAGGGACACGACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG9290.12010358125867002No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTCGTGTCCCTTGGCCGAAGGTGACAGGAGGCCAATCACTACGTTG9000.1163543844271292No Hit
CTGTTGGTACCAGGCTAAGTAGGTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTCTGCCCTGGAGA8920.11532012323222138No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8890.11493227528413094No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8770.11338088349176921No Hit
GTGTTAGCACCTACTTAGCCTGGTACCAACAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCCATGATGCATCC8730.11286375289431533No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8700.11247590494622489No Hit
CAGTAATACACAGCCGTGTCCTCAGCTGTCAGGCTGTTCATTTGCAGAAA8690.1123466222968614No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8680.11221733964749793No Hit
CTGCTACTCTGGCTCCCAGATGTTACCGGAGAAGTTGTGTTGACACAGTC8520.11014881725768229No Hit
GGTGACAGGAGGCCAATCACTACGTTGCTGACAGTAATAAACTGCAGAAT8510.11001953460831881No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8510.11001953460831881No Hit
CTCCAGGCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGCATAGGTAC8480.10963168666022838No Hit
TCCATGATGCATCCCACAGGGCCCCTGGCGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT8450.10924383871213797No Hit
TTATTACTGTCAGCAACGTAGTGATTGGCCTCCTGTCACCTTCGGCCAAGGGACACGACTGGAGATCAAACGAA8270.10691675102359538No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8260.10678746837423189No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8110.10484822863377975No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGTC8080.10446038068568932No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7840.10135759710096588No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC150.001862157373.2095263
ACGGTAG150.001990829671.9869158
CGTCCGT150.002413645268.5750238
CTAGTAC252.923688E-453.7633823
ACGGGTT350.001528649638.42976416
GCACATT400.001941165236.60475563
GGTATCA9350.035.926471
TCTGGAG6300.034.81852370
GTATCAA19600.032.220181
ACCAGGT1651.7098846E-632.08979870
ATGATCA1254.0152157E-430.25609470
AATCGTT802.8166523E-630.10889639
GTTTCGG450.00507747130.08261730
ATAGCGG754.114359E-529.28380663
CGCGAGG500.006525384328.57693355
GCCCCTT605.7338213E-428.47518336
GACAGGT2150.028.1229081
AGTATTA500.007269148227.95218546
AACGCAG23600.027.9086486
TATCAAC23100.027.483752
Produced by FastQC (version 0.11.5)