FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1226020
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69750.5689140470791667No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51670.42144500089721215No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC45080.3676938385996966No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44140.3600267532340419No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37390.30497055512960636No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31880.2600283845288005No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31510.2570104892252981No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA31360.25578701815631066No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30360.24763054436306095No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28720.23425392734213146No Hit
ATAGGGGACAGTGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAA28370.23139916151449408No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC28260.2305019493972366No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28210.2300941257075741No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27560.22479241774196182No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27160.22152982822466188No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26130.2131286602176147No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25770.21019232965204482No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25150.20513531590023001No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24880.2029330679760526No Hit
ATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA24270.19795761896217026No Hit
CTATAGGGGACAGTGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGT23490.19159556940343553No Hit
ACCTTGAAGGGACCCCACTTTCTAAACTAGACGCCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG23330.19029053359651554No Hit
GACTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGT23310.19012740412065054No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTTCAACTTATTACTGCCAACACTATAATAATAA23050.18800672093440565No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22970.18735420303094566No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22550.18392848403778078No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGAAGGGACCCCA22410.18278657770672582No Hit
GTGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAG22390.18262344823086082No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22240.18139997716187337No Hit
CTATAATAATAACTTCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT21820.1779742581687085No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21250.17332506810655618No Hit
TTATTATAGTGTTGGCAGTAATAAGTTGAAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC21130.17234629125136622No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20440.1667183243340239No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20420.16655519485815892No Hit
CCTACTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACACTGTCCCCTATAGATGCTGACAGGGTGGAAGGAGA19700.16068253372701913No Hit
CTTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTT19700.16068253372701913No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19470.1588065447545717No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT19170.1563596026165968No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18660.15219980098203945No Hit
GGCCAAAAGTGTACATGAAGTTATTATTATAGTGTTGGCAGTAATAAGTTGAAAAATCATCAGGCTGCAGGCTG18460.1505685062233895No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18320.14942659989233456No Hit
TGGTGACACTGTCCCCTATAGATGCTGACAGGGTGGAAGGAGACTGGGTC17900.14600088089916965No Hit
ATTATAGTGTTGGCAGTAATAAGTTGAAAAATCATCAGGCTGCAGGCTGC17840.1455114924715747No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17750.14477740983018222No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG17540.14306455033359977No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17410.1420042087404773No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17330.14135169083701735No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17320.14127012609908485No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17230.14053604345769238No Hit
CCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCACCTACTAATAGTCTGA17210.14037291398182739No Hit
CTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACACTGTCCCCTATAGA17200.1402913492438949No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16970.13841536027144744No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16720.13637624182313504No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16580.13523433549208005No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC16500.1345818175886201No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16490.1345002528506876No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16450.1341739938989576No Hit
CACTATAATAATAACTTCATGTACACTTTTGGCCAGGGGACCAAGCTGGA16170.13189018123684768No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15970.13025888647819775No Hit
GTCCCCTATAGATGCTGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG15630.12748568538849284No Hit
CCTTCCACCCTGTCAGCATCTATAGGGGACAGTGTCACCATCACTTGCCG15370.12536500220224792No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15180.12381527218153048No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACATGAAGTT15030.12259180111254303No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14830.12096050635389309No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14740.1202264237125006No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14540.11859512895385066No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14160.11549566891241579No Hit
GTCTCCTTCCACCCTGTCAGCATCTATAGGGGACAGTGTCACCATCACTT13940.11370124467790085No Hit
GATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCTTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA13870.11313029151237337No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13530.11035709042266847No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT13490.11003083147093848No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13350.10888892513988353No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCACCTACTA13190.10758388933296358No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12860.10489225298119116No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12740.1039134761260012No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12670.10334252296047372No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCTTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT12670.10334252296047372No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12650.10317939348460872No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12460.1016296634638913No Hit
GCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGAAGGGACCCCACTTTCTAAACTAGA12370.10089558082249882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3050.039.754451
TAAGTCA2350.037.48626770
GTATCAA24450.036.367061
AATGGTA4850.036.32689770
TAGCGCG400.002844116433.8646729
AACGCAG28550.031.4906716
CAACGCA28500.031.4277675
TCAACGC28400.031.422434
ATCAACG28300.031.4170343
TATCAAC28200.031.412862
CGCATAA450.00458205130.7221741
TCGGTTT1000.005712955729.36423970
ACGCAGA30600.029.270967
CGCAGAG30950.028.8311548
GACAGGT1302.6193447E-1028.4991841
AGAGTAC31250.028.22535711
GCAGAGT32000.028.0955839
CAGAGTA31900.028.07695610
TATAGGT606.24674E-428.0627984
TGTTAGA19050.026.97502370
Produced by FastQC (version 0.11.5)