FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378599
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT49090.35608614252585413No Hit
GTATTACTGTCAGACGTGGGGCATTGGCATTCAGGTCTTCGGAACTGGGACCGAGGTGACCGTCCTAGGTCAGCC44210.3206878867603995No Hit
GAGTATTACTGTCAGACGTGGGGCATTGGCATTCAGGTCTTCGGAACTGGGACCGAGGTGACCGTCCTAGGTCAG43240.31365175805292184No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC43030.3121284724564576No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA42000.30465711929284733No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG39020.2830409713049262No Hit
CAGTAATACTCAGCCTCATCCTCAGACTGGAGACTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGC38000.2756421555506714No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC37510.2720878224922548No Hit
GACCTAGGACGGTCACCTCGGTCCCAGTTCCGAAGACCTGAATGCCAATGCCCCACGTCTGACAGTAATACTCAG33870.2456842054868747No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA33620.24387077025298873No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33570.24350808320621153No Hit
GTAATACTCAGCCTCATCCTCAGACTGGAGACTGGAGATGGTGAGGTAGC30770.22319760858668836No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT30140.21862775179729566No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT30110.21841013956922936No Hit
GGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC29870.21666924174469882No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCT29410.21333252091434857No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC28490.2066590792536481No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC28080.20368504547007504No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTCGGTCCCAGTTCCGAAGACCTG27540.19976802536488128No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26680.19352980816031348No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT26220.19019308732996326No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTCACCTCGGTC25970.18837965209607724No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG25260.18322949603184102No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG25070.18185128525408767No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATC24190.17546799323080894No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG23980.17394470763434472No Hit
GTACTTGATGAAGCTTGACAGTGATGGCAGGCACAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAG23350.169374850844952No Hit
CAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGG23250.16864947675139763No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT23000.1668360415175116No Hit
CCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAG22500.16320917104973962No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG22460.16291902141231787No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT21800.15813155239485882No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA21020.15247363446513454No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG20740.15044258700318222No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGTCTCCAGTCTGAGGAT20320.14739601581025374No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG20180.14638049207927759No Hit
GTATATTACTGTGCCAAGTTACCCAGGGGCGGGGGGCCAACACCTGACACCGGAGGGTACTGGGGCCAGGGAACC20180.14638049207927759No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA19970.14485720648281336No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19720.14304377124892734No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC19430.1409401863776196No Hit
TCCTCAGACTGGAGACTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC19170.13905421373437815No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18990.13774854036598025No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC18190.13194554761754507No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC17760.12882643901526114No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC17280.12534464336620002No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTG17180.12461926927264565No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTACCACCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGA16590.12033956212067468No Hit
GTCTGAGGATGAGGCTGAGTATTACTGTCAGACGTGGGGCATTGGCATTC16530.11990433766454205No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG16250.11787329020258974No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16150.11714791610903533No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT16040.11635000460612549No Hit
CTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT15970.11584224274063741No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15910.11540701828450478No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT15890.1152619434657939No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC15820.11475418160030583No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC15430.11192522263544366No Hit
ATCAAGTACCGAGGGCCCTTCTCTGGCTGTTGCTGATGCCATGCGATGGC15290.11090969890446752No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG15150.10989417517349134No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGC15130.10974910035478047No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA15120.10967656294542502No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15030.10902372626122607No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14750.10699267879927377No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC14640.10619476729636391No Hit
GCCTCATCCTCAGACTGGAGACTGGAGATGGTGAGGTAGCGCTCAGCCCC14590.10583208024958672No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14590.10583208024958672No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14560.1056144680215204No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG14500.10517924356538776No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG14360.10416371983441161No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14200.10300312128472458No Hit
GGATGAGGCTGAGTATTACTGTCAGACGTGGGGCATTGGCATTCAGGTCTTCGGAACTGGGACCGAGGTGACCGT13950.10118968605083857No Hit
CCCTTGCTGTGCCTGCCATCACTGTCAAGCTTCATCAAGTACCGAGGGCC13800.10010162491050696No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)