FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001445
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTACGAAGTTT54740.546610148335655No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43410.4334736306037775No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36820.3676687187014764No Hit
TTCCATCACTGTGCAAGAGACTCTGACTAGACTTGCAGGAGATGGAGGCC33090.3304225394305229No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28140.2809939637224211No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27840.27799829246738467No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26920.2688115672852728No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24240.24205023740694695No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21790.2175855888241491No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21470.2143902061521102No Hit
GTGCAGATATTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCCCT21000.20969698785255309No Hit
CTCTTGCACAGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGCCAGGCCAGCCTCCACAGCTCCTGAT20940.20909785360154576No Hit
GTTCTCTGGAGTGCCAGAAAGGTTCAGTGGCAGCGGGTCAGGGACAGATT20610.20580261522100562No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20460.20430477959348742No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20310.20280694396596918No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19420.193919785909361No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19410.19381993020085977No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGATGA19240.1921223831563391No Hit
GGATACCTGGATGCAGTGCAGATATTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCCCTGGACAGCCGG19000.18972584615230992No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17990.17964041959368712No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17850.1782424396746701No Hit
GTGATATAGAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGGTACA17430.17404849991761903No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC16510.1648617747355072No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGTCTCTTG16260.16236538202297682No Hit
GTTTACAGATTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA16180.16156653635496707No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16070.1604681235614537No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACTCACTGTACCTG15920.15897028793393547No Hit
TTATTACTGCATGCAAAGTTTACAGATTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG15910.15887043222543423No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA15610.15587476097039776No Hit
CTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTA15570.15547533813639292No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAATCTGTCCCTGACCCGCTGCCA15410.15387764680037347No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15360.15337836825786738No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15110.15088197554533697No Hit
CCTGGATGCAGTGCAGATATTGTGATGACCCAGACTCCACTCTCTCTGTC15090.15068226412833455No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGATGACCCAGACTCCACTCTCT15050.15028284129432967No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15020.14998327416882604No Hit
GTTTATTACTGCATGCAAAGTTTACAGATTTACACTTTTGGCCAGGGGAC14620.14598904582877742No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14550.1452900558692689No Hit
GTGCAAGAGACTCTGACTAGACTTGCAGGAGATGGAGGCCGGCTGTCCAGGGGTGACGGACAGAGAGAGTGGAGT14490.1446909216182616No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGTCTCTTGCACAGTGATGGAAAGACCTATTTGT14270.14249409603123486No Hit
CCAGAGAACCGGTTGGAAACTTCGTAGATCAGGAGCTGTGGAGGCTGGCC14130.14109611611221784No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG14060.14039712615270933No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13960.13939856906769718No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13860.13840001198268503No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13850.1383001562741838No Hit
GTATATGGTATCACTATCACTAGTACCACCAATGTATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGAC13510.13490506218514245No Hit
AGCCTCCACAGCTCCTGATCTACGAAGTTTCCAACCGGTTCTCTGGAGTGCCAGAAAGGTTCAGTGGCAGCGGGT13270.13250852518111328No Hit
GCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCC13100.13081097813659262No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13050.13031169959408653No Hit
GTATTGGTACCTGCAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTACG12900.12881386396656833No Hit
GGTCAGGGACAGATTTCACACTGAAAATCAGCCGGGTGGAGGCTGAGGAT12890.12871400825806711No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG12570.1255186255860282No Hit
GTCCAGGGGTGACGGACAGAGAGAGTGGAGTCTGGGTCATCACAATATCT12520.1250193470435221No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT12490.12471977991801847No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12360.12342165570750266No Hit
CTATATCACTGTGTGAGAGATCTGACCTACTGGGGCCAGGGAACCCTCGT12100.12082540728647105No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGATGACCCAGACTCCACT12010.1199267059099601No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC11980.11962713878445647No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11670.11653161182091877No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11580.11563291044440784No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11540.11523348761040297No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11460.11443464194239325No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11450.11433478623389201No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11190.11173853781286042No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11170.11153882639585798No Hit
GTGGAGGCTGGCCTGGCTTCTGCAGGTACCAATACAAATAGGTCTTTCCA11060.11044041360234462No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11010.10994113505983855No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10960.10944185651733246No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10830.10814373230681666No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10800.107844165181313No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10790.10774430947281179No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10770.10754459805580935No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAATCTGTCCCTGACCCGCTGCCA10650.10634632955379476No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10570.10554748388578504No Hit
GTGTAAATCTGTAAACTTTGCATGCAGTAATAAACCCCAACATCCTCAGC10480.10464878250927409No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10430.10414950396676803No Hit
GATTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG10370.10355036971576072No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10330.10315094688175587No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10190.10175296696273883No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10160.1014533998372352No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)