FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001445
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTACGAAGTTT44930.44865169829596235No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42440.42378762687915966No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35390.35338935238580255No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31240.31194923335779795No Hit
TTCCATCACTGTGCAAGAGACTCTGACTAGACTTGCAGGAGATGGAGGCC29930.2988681355441387No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28130.2808941080139199No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28040.2799954066374089No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAATCTGTCCCTGACCCGCTGCCA23310.2327636565163339No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22830.22797058250827557No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22630.22597346833825122No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22240.22207909570670384No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19310.1928213731158476No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18980.18952613473530747No Hit
GTGCAGATATTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCCCT18710.18683003060577463No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18460.18433363789324425No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18190.18163753376371142No Hit
GTTCTCTGGAGTGCCAGAAAGGTTCAGTGGCAGCGGGTCAGGGACAGATT18010.17984013101068955No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17890.17864186250867498No Hit
CTCTTGCACAGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGCCAGGCCAGCCTCCACAGCTCCTGAT17470.1744479227516239No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17080.1705535501200765No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16790.16765773457354124No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16600.16576047611201813No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC16480.16456220761000354No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGATGA16430.16406292906749748No Hit
GGATACCTGGATGCAGTGCAGATATTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCCCTGGACAGCCGG16130.16106725781246098No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15700.15677346234690873No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15530.15507591530238807No Hit
GTGATATAGAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGGTACA15430.1540773582173759No Hit
TTATTACTGCATGCAAAGTTTACAGATTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG15230.1520802440473516No Hit
GTTTACAGATTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA14880.148585294249809No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14870.1484854385413078No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACTCACTGTACCTG14730.1470874586222908No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGTCTCTTGCACAGTGATGGAAAGACCTATTTGT14670.1464883243712835No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA14580.14558962299477254No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14560.14538991157777012No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14480.1445910659097604No Hit
GTTTATTACTGCATGCAAAGTTTACAGATTTACACTTTTGGCCAGGGGAC14190.14169525036322514No Hit
GTGCAAGAGACTCTGACTAGACTTGCAGGAGATGGAGGCCGGCTGTCCAGGGGTGACGGACAGAGAGAGTGGAGT14160.1413956832377215No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAATCTGTCCCTGACCCGCTGCCA14140.14119597182071905No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13960.13939856906769718No Hit
CCTGGATGCAGTGCAGATATTGTGATGACCCAGACTCCACTCTCTCTGTC13610.13590361927015462No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG13460.1344057836426364No Hit
CCAGAGAACCGGTTGGAAACTTCGTAGATCAGGAGCTGTGGAGGCTGGCC13450.13430592793413518No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13430.13410621651713275No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG13420.13400636080863154No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGATGACCCAGACTCCACTCTCT13260.13240866947261207No Hit
GTCCCTGACCCGCTGCCACTGAACCTTTCTGGCACTCCAGAGAACCGGTT13140.1312104009705975No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12940.12921328680057317No Hit
CTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTA12590.12571833700303062No Hit
GTATATGGTATCACTATCACTAGTACCACCAATGTATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGAC12590.12571833700303062No Hit
TCTGTAAACTTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAAT12520.1250193470435221No Hit
GTGTAAATCTGTAAACTTTGCATGCAGTAATAAACCCCAACATCCTCAGC12400.12382107854150752No Hit
GGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAAGTTTACAGA12010.1199267059099601No Hit
GTCCAGGGGTGACGGACAGAGAGAGTGGAGTCTGGGTCATCACAATATCT11940.1192277159504516No Hit
GGTCAGGGACAGATTTCACACTGAAAATCAGCCGGGTGGAGGCTGAGGAT11870.11852872599094309No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11770.11753016890593093No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11710.11693103465492363No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11550.11533334331890419No Hit
GTGGAGGCTGGCCTGGCTTCTGCAGGTACCAATACAAATAGGTCTTTCCA11370.1135359405658823No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11260.11243752777236893No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC11220.11203810493836405No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11190.11173853781286042No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11140.11123925927035434No Hit
AGCCTCCACAGCTCCTGATCTACGAAGTTTCCAACCGGTTCTCTGGAGTGCCAGAAAGGTTCAGTGGCAGCGGGT11060.11044041360234462No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10790.10774430947281179No Hit
GCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCC10790.10774430947281179No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10730.1071451752218045No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10600.1058470510112887No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10410.10394979254976558No Hit
GTATTGGTACCTGCAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTACG10340.10325080259025708No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10340.10325080259025708No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10260.10245195692224736No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATGCAGTGCAGATATTGTGATGACCCAGACTCCACT10150.10135354412873399No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10110.10095412129472912No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10060.10045484275222304No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATTA150.002283901769.5387646
CCCCGTT200.006608229653.17696455
CCCGTTG200.00713584652.15406846
AATATCG200.00787797650.86515422
GTATCAA15050.037.1177671
GTGGTAT2950.034.429731
GGTAACA400.002725503234.15960334
CGTTATA400.002804517333.9610727
GAGTTTA2300.030.9119111
ACAGAGA17450.030.36317670
TATCAAC18550.030.1189292
AGTACCG450.005063753630.100155
AGTTTAT2500.029.7976572
ATCAACG18800.029.1780723
CAACGCA18750.029.0767445
TCAACGC18800.028.9979614
TACTTAG500.006180162528.89758762
AACGCAG19000.028.8723816
ACGGGGC2000.028.80773216
TCGGTGA500.00677261328.36104655
Produced by FastQC (version 0.11.5)