FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017918
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT21180.20807177002469748No Hit
GTGATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATCTGCAGATACA19300.18960269884214642No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14230.13979515049345823No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12130.11916480502358737No Hit
GTGTATCACTGTGCGAGATCGTATTGTTATGATAAAAGTGGTATCCCATA11790.11582465385227493No Hit
GTACACAATTTCCTCATACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA11030.10835843358698834No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAC200.00760147351.32700334
ATTCGCG200.007789024651.01154326
CGTATGC5300.041.74947441
ACCGTAC4900.040.97826427
TCGTATG5400.040.89864340
GACCGTA5050.040.4051826
CGTACTA5200.039.9781729
ATGCCGT5600.039.05951344
GTACTAG5450.038.79638330
GTATCAA29500.037.7989351
GGTATCA11900.037.7098431
ATCTCGT4750.037.6066937
CCCACGA5800.037.49623519
CACGAGA5900.036.29367421
ACTAGAT5350.035.7462432
CCGTCTT6000.035.44948647
ACGAGAC6050.035.3938322
CTAGATC4850.035.24490433
TCTCCGA6200.035.07195311
GAGACCG6100.034.55524
Produced by FastQC (version 0.11.5)