FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017918
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47740.46899652034839745No Hit
GTGATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATCTGCAGATACA18120.17801040948288566No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13110.12879229957619376No Hit
GTGTATCACTGTGCGAGATCGTATTGTTATGATAAAAGTGGTATCCCATA12460.12240671645456706No Hit
GTACACAATTTCCTCATACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA11360.11160034501796803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12550.037.7895431
GTATCAA30900.037.054891
ATCAACG35700.031.8907783
TCAACGC36150.031.406244
TATCAAC36400.031.1813412
CAACGCA36500.031.1050855
AACGCAG36950.030.9097086
CGCAGAG38450.029.880158
AGAGTAC39100.029.46575511
GCAGAGT40400.028.6056949
CAGAGTA40350.028.55714610
ATACGTA1405.7661964E-1026.62441411
GTGCTAG1751.0913936E-1125.1647511
ACGCAGA45650.025.0189197
TCTGGAG12650.025.00046270
ACGTACC1501.2842065E-924.8470113
AATCGTC7450.024.75565563
AGTACGG34800.024.14599613
ATCGTCT7700.024.0363864
TCGTCTC7900.023.99713765
Produced by FastQC (version 0.11.5)