FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1230204
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46780.3802621353856758No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAACCTGCAGTCTGGGGATT35700.2901957724084786No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34000.27637692610331294No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31760.25816856391297704No Hit
GAGTTCACTCTCACCATCAGCAACCTGCAGTCTGGGGATTTTGCAGTTTACTTCTGTCAGCAGTATGATAATTGG30700.24955210680505024No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30200.24548774024470738No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29880.24288654564608797No Hit
GTCCCAGACCCACCGCCACTGAATGTGGCTGGGATGCCGTTGGCCCTGGT29680.24126079902195083No Hit
GTATGATAATTGGCCTCCCACCTTCGGCCAAGGGACACGACTAGAGATCAAACGAACTGTGGCTGCACCAT28350.2304495839714389No Hit
CTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGTCA26680.2168745996598938No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24290.19744692750145507No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAAGAACAACTTAGCCTGGTACCAACAGAAGCCTGCCCAGGCTCCCAGGCT23270.18915561971835565No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAAGAA22930.1863918504573225No Hit
TTATCATACTGCTGACAGAAGTAAACTGCAAAATCCCCAGACTGCAGGTTGCTGATGGTGAGAGTGAACTCTGTC22330.18151461058491114No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22020.17899470331749856No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21640.175905784731638No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAAGAACAACTTAGCCTGGTA21620.1757432100692243No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21360.17362973945784602No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21180.17216656749612258No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20830.1693215109038826No Hit
GAGTGAACTCTGTCCCAGACCCACCGCCACTGAATGTGGCTGGGATGCCG20740.1685899249230209No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20510.16672031630526318No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20310.16509456968112607No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC19860.1614366397768175No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19430.15794128453492268No Hit
TCTATGATGCGTTCACCAGGGCCAACGGCATCCCAGCCACATTCAGTGGCGGTGGGTCTGGGACAGAGTTCACT19310.15696583656044039No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18750.1524137460128564No Hit
GTGCAGCCACAGTTCGTTTGATCTCTAGTCGTGTCCCTTGGCCGAAGGTG18730.1522511713504427No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18280.14859324144613414No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18150.147536506140445No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17300.1406270829878622No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17060.1386761870388976No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16850.13696915308355362No Hit
CGACTAGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT16600.13493696980338218No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15810.12851527063804052No Hit
ACAGAAGCCTGCCCAGGCTCCCAGGCTCCTCATCTATGATGCGTTCACCAGGGCCAACGGCATCCCAGCCACATT15230.12380060542804283No Hit
CCCTTAGACTCTCCTGCACAGTCTCTGGATTGACTTTCAGTGACGCCTGG15050.1223374334663194No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14910.12119941082942341No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14730.11973623886769999No Hit
CTGCTACTCTGGCTCCCAGATGTCACTGGAGAAGTCGTGATGACGCAGTC14710.11957366420528627No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14520.118029204912356No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14480.11770405558752858No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14410.11713504426908057No Hit
GTTTACTTCTGTCAGCAGTATGATAATTGGCCTCCCACCTTCGGCCAAGGGACACGACTAGAGATCAAACGAA14180.11526543565132288No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14070.11437127500804743No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13950.11339582703356517No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13860.11266424105270346No Hit
GAATGTGGCTGGGATGCCGTTGGCCCTGGTGAACGCATCATAGATGAGGAGCCTGGGAGCCTGGGCAGGCTT13630.11079463243494575No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13620.1107133451037389No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTAGTCGTGTCCCTTGGCCGAAGGTGGGAGGCCAATT13170.10705541519943035No Hit
CATCATAGATGAGGAGCCTGGGAGCCTGGGCAGGCTTCTGTTGGTACCAGGCTAAGTTGTTCTTAACACTCTGA12950.10526709391287949No Hit
CCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAAGAACAACTTAGC12930.10510451925046578No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGTCACTGGAGAAGT12900.1048606572568452No Hit
GAAGTAAACTGCAAAATCCCCAGACTGCAGGTTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACCGCCA12890.10477936992563834No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12750.10364134728874236No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12600.1024220373206395No Hit
CATCTATGATGCGTTCACCAGGGCCAACGGCATCCCAGCCACATTCAGTGGCGGTGGGTCTGGGACAGAGTTCA12580.10225946265822577No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12330.10022727937805437No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)