FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416777
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT23460.5628909464773728No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14890.3572653961231066No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14780.35462609501004133No Hit
GTATAATATTGCTGACAGTGATAAACTGCCACATCTTCAGGCTGCAGGCTGTTGATGGTGAGAGTGAAATGTGTC13670.32799314741456465No Hit
ATACTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA12740.30567905618592195No Hit
GGGTAGTATAATATTGCTGACAGTGATAAACTGCCACATCTTCAGGCTGC10540.25289303392461676No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT10500.25193328806532034No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10110.24257576593717983No Hit
GTAATAGACGGCTGTGTCTTCGATTTCCAGGCTGTTCATTTGCAGATATA9370.22482046754019538No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9190.2205016111733613No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGACCCGGGATCCCT9120.2188220559195925No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8920.2140233266231102No Hit
ATTTTATACAGTTTCAACAATAAAAATTACTTAGCTTGGTACCAACAGAA8710.2089846608618038No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8650.20754504207285912No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8610.20658529621356264No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGTTGGTACCAAGCTAAGTAATTTTTATTGTTGAAACTGTATAAAATACT8340.20010701166331157No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8320.19962713873366333No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8020.19242904478893988No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7960.1909894259999952No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7890.1893098707462264No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7830.18787025195728171No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7550.1811520309422065No Hit
CATTTCACTCTCACCATCAACAGCCTGCAGCCTGAAGATGTGGCAGTTTATCACTGTCAGCAATATTATACTACC7520.18043222154773417No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGTTTCGCTTTCGGTAACGCCTGG7110.17059482648994548No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6690.16051749496733264No Hit
GTTTATCACTGTCAGCAATATTATACTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA6570.15763825738944326No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6310.1513999093040163No Hit
GTCTATTACTGTGCCGCAGTCAAGGCATACTATGATAACGATGGTTATTTCTCAAACTGGTTCGACCCCTGGGGC6210.14900054465577514No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGA5990.14372194242964462No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA5960.14300213303517229No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5810.13940308606281057No Hit
GTATAAAATACTCTGACTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA5710.13700372141456943No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5650.13556410262562474No Hit
CTCCTGTGCAGCCTCTGGTTTCGCTTTCGGTAACGCCTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGC5590.13412448383668005No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5520.13244492858291124No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAGTATTTTATA5500.13196505565326302No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA5350.12836600868090128No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5270.12644651696230838No Hit
ATTCAGTGGCAGCGGGTCTGGGACACATTTCACTCTCACCATCAACAGCC5210.1250068981733637No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC5140.12332734291959489No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAGTATTTTATACAGTTTCAA5010.12020816887688139No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG4990.11972829594723317No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA4970.11924842301758495No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4940.1185286136231126No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT4890.11732893129899204No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT4840.11612924897487147No Hit
GTACCAACAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGACCCGGGATCCCTGA4830.11588931251004735No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC4790.11492956665075088No Hit
CACCATCAACAGCCTGCAGCCTGAAGATGTGGCAGTTTATCACTGTCAGCAATATTATACTACCCCGCTCACTTT4750.11396982079145443No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC4690.11253020200250974No Hit
GGACTGAACACAGAGGACTCCCCATGGAGTTTGGGCTGAGCTGGATTTTC4670.11205032907286151No Hit
GCCTGAAGATGTGGCAGTTTATCACTGTCAGCAATATTATACTACCCCGC4640.11133051967838917No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT4610.11061071028391682No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT4600.1103707738190927No Hit
CTCCAGGCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAGGCGTTAC4540.10893115503014801No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC4440.10653179038190687No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG4430.10629185391708275No Hit
GTGATAAACTGCCACATCTTCAGGCTGCAGGCTGTTGATGGTGAGAGTGAAATGTGTCCCAGACCCGCTGCCA4410.10581198098743452No Hit
GGCCTGGAGTGGGTCGGCCGTATTAAGGGCAAAACTGATGGTGAGACAAT4350.10437236219848983No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG4310.1034126163391934No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA4220.10125318815577636No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA4220.10125318815577636No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA4190.10053337876130401No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATTA150.002638820867.044154
TACTGTT200.00824127250.283117
TGTCGTA552.739398E-949.86618838
ATGTCGT552.7903297E-949.7623937
TGGTATC750.049.165712
GATGTCG606.1390892E-945.52077536
GGTATCA2700.044.706831
ACGTACC307.2623603E-444.70146613
TCGTATG3350.044.27668840
GTCGTAT551.5029764E-743.7725639
TGCTTGA3450.043.765755
GTGGTAT851.8189894E-1243.3919221
ATCTCGT2950.042.90952337
CCGTCTT3600.042.8854547
CGTCTTC3450.042.81810848
CGTATGC3500.042.5103241
ATGCCGT3450.042.4644
GCGATCT3050.042.37013234
CTGCTTG3400.042.16438354
TATACAC4500.041.716363
Produced by FastQC (version 0.11.5)