FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1110565
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36090.3249697226186671No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT34220.30813144660600683No Hit
GCCTTGGGCTGACCTAGAACGGTCAGCTTGGTCCCTCCGCCGAACAGCAC30340.27319427498615567No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG25660.23105356282612904No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC25360.22835223512356323No Hit
CCTTAGACCTCCCAGACCGATTCTCTGGCTCCTCCTCAGGAAACACAGCTTCCTTGACCATCACTGGGGCTCAGG24510.22069847329962677No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23510.2116940476244074No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21090.18990333749037652No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19870.1789179381666089No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18930.17045377803190268No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18910.1702736895183983No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGAACGGTCAGCTTGGTCCCTC17940.1615393966134355No Hit
CTATTACTGTAACTCCCGGGACAGCAGTGGTGACCATGTGCTGTTCGGCG17740.15973851147839163No Hit
TTCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17720.15955842296488723No Hit
GACCTAGAACGGTCAGCTTGGTCCCTCCGCCGAACAGCACATGGTCACCA17490.1574874050595868No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16830.151544484113942No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16480.14839293512761523No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16270.14650200573581915No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15880.1429902797224836No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15680.14118939458743973No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15420.1388482439118827No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC15010.13515642938504274No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14890.13407589830401642No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14760.13290532296623792No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14650.13191483614196378No Hit
GACTATTACTGTAACTCCCGGGACAGCAGTGGTGACCATGTGCTGTTCGG14640.13182479188521157No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14470.1302940395204243No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14360.12930355269615015No Hit
CTCCAGCCCCTTCCCTGGTGCCTGGCGGACCCAACTCATAGCGTGGCGACCGAAGGTGAATCCAGAGGCCTCACA14310.1288533314123892No Hit
GCATAGTACTTATAACTTCCACTGCTACTAACAGATGCGACCCACTCCAGCCCCTTCCCTGGTGCCTGGCGGACC14260.12840311012862823No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14240.12822302161512383No Hit
GTTCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14040.12642213648007997No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13770.12399094154777074No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13740.12372080877751414No Hit
CTCCTCACTCTTTGCATAGGTTCTGTCGTTTCTTCTCAGCTGACTCAGGGCCCTGCTGTGTCTGTGGCCTTGGGA13700.12336063175050536No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13420.12083939256144396No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13320.11993894999392202No Hit
GTACTATGCAGACTCACTGAGGGGCCACCTCACCATCTCCAGAGACAACG13170.1185882861426391No Hit
CCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTAGCTATTTTAGAAGGT13010.11714757803460402No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12990.11696748952109962No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12860.1157969141833211No Hit
GGTCTAAGGAGCGGGAATTATTAGAATAGATGACAAGTAGAGGGGCCTGGCCTGGCTTCTGCTGATACCAGGTTG12820.11543673715631232No Hit
CTCTTTGCATAGGTTCTGTCGTTTCTTCTCAGCTGACTCAGGGCCCTGCT12810.11534669289956012No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12790.11516660438605575No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12780.11507656012930355No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12600.11345576350776407No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12600.11345576350776407No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGGTGATCAGCACTGAGCACCGAGGATTCACCATGGAA12580.11327567499425967No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12570.1131856307375075No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12440.11201505539972897No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12410.11174492262947239No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12180.10967390472417192No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12100.10895355067015437No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12040.10841328512964123No Hit
GGCCTGTTGGGTCCCTGAGACTCTCGTGTGAGGCCTCTGGATTCACCTTC11790.10616217871083637No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC11710.10544182465681882No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11670.10508164762981005No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCC11600.10445133783254469No Hit
GTAGAGGGGCCTGGCCTGGCTTCTGCTGATACCAGGTTGCAGAATAGGTT11530.10382102803527934No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG11500.10355089526502277No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTCGTTTCTTCTCAGCTGACTCAGGG11450.1031006739812618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG5650.085.2380570
TTATACA7750.043.212852
TATACAC7700.043.0541233
AAAGGGG5750.041.66504368
CGTATGC7850.041.37008341
ATGCCGT8000.041.23144
TCTCGTA7050.040.9045238
TCGTATG7950.040.76161240
CTTATAC8000.040.6141
GTATGCC8100.040.58506842
CTTGAAA8200.040.53808257
CCGTCTT8000.039.72995847
GCCGTCT8000.039.68015746
GTAGTTT1253.2631033E-739.66076770
ACTAGGC8050.039.5618627
ACGAGAC8250.039.3726322
ATCTCGT7050.039.3698937
TACACAT8650.039.107795
AAAAGGG5750.038.97722267
CGAGACT8250.038.96425623
Produced by FastQC (version 0.11.5)